Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Methylococcus capsulatus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the soluble methane monooxygenase contains no copper | Methylococcus capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + H+ + O2 | Methylococcus capsulatus | - |
methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | Methylococcus capsulatus Bath | - |
methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | Methylococcus capsulatus Bath. | - |
methanol + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
- |
- |
Methylococcus capsulatus | P22869 and P18798 and P11987 and P18797 and P22868 and P22867 | P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
Methylococcus capsulatus Bath | - |
- |
- |
Methylococcus capsulatus Bath. | P22869 and P18798 and P11987 and P18797 and P22868 and P22867 | P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | transcriptomic profiling of soluble MMO is used to investigate the gene expression pattern in Methylococcus capsulatus during methanotrophic growth in the presence and absence of copper, overview | Methylococcus capsulatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NADH + H+ + O2 | - |
Methylococcus capsulatus | methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD+ + H2O | - |
? | |
methane + NADH + H+ + O2 | - |
Methylococcus capsulatus Bath. | methanol + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sMMO | - |
Methylococcus capsulatus |
soluble methane monooxygenase | - |
Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme expresses the soluble enzyme form under copper limitation, and the membrane-bound particulate MMO at high copper-to-biomass ratio, analysis of the mechanism of the copper switch. Transcriptomic profiling of particulate MMO, EC 1.14.18.3, and soluble MMO, using Methylococcus capsulatus DNA microarrays. 137 ORFs are found to be differentially expressed between cells producing sMMO and pMMO, while only minor differences in gene expression are observed between the pMMO-producing cultures. Of these, 87 genes are upregulated during sMMO-producing cells, i.e. during copper-limited growth. Major changes takes place in the respiratory chain between pMMO-and sMMO-producing cells, and quinone are predominantly used as the electron donors for methane oxidation by pMMO. Proposed pathway of methane oxidation in Methylococcus capsulatus cells producing either sMMO or pMMO, overview | Methylococcus capsulatus |
metabolism | the high number of up-regulated genes in cells producing soluble methane monooxygenase shows that Methylococcus capsulatus is highly adapted to copper-limited growth | Methylococcus capsulatus |