Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.25 extracted from

  • Larsen, O.; Karlsen, O.A.
    Transcriptomic profiling of Methylococcus capsulatus (Bath) during growth with two different methane monooxygenases (2016), MicrobiologyOpen, 5, 254-267 .
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Methylococcus capsulatus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the soluble methane monooxygenase contains no copper Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NADH + H+ + O2 Methylococcus capsulatus
-
methanol + NAD+ + H2O
-
?
methane + NADH + H+ + O2 Methylococcus capsulatus Bath
-
methanol + NAD+ + H2O
-
?
methane + NADH + H+ + O2 Methylococcus capsulatus Bath.
-
methanol + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus P22869 and P18798 and P11987 and P18797 and P22868 and P22867 P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD)
-
Methylococcus capsulatus Bath
-
-
-
Methylococcus capsulatus Bath. P22869 and P18798 and P11987 and P18797 and P22868 and P22867 P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD)
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information transcriptomic profiling of soluble MMO is used to investigate the gene expression pattern in Methylococcus capsulatus during methanotrophic growth in the presence and absence of copper, overview Methylococcus capsulatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NADH + H+ + O2
-
Methylococcus capsulatus methanol + NAD+ + H2O
-
?
methane + NADH + H+ + O2
-
Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
?
methane + NADH + H+ + O2
-
Methylococcus capsulatus Bath. methanol + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
sMMO
-
Methylococcus capsulatus
soluble methane monooxygenase
-
Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylococcus capsulatus

General Information

General Information Comment Organism
metabolism the enzyme expresses the soluble enzyme form under copper limitation, and the membrane-bound particulate MMO at high copper-to-biomass ratio, analysis of the mechanism of the copper switch. Transcriptomic profiling of particulate MMO, EC 1.14.18.3, and soluble MMO, using Methylococcus capsulatus DNA microarrays. 137 ORFs are found to be differentially expressed between cells producing sMMO and pMMO, while only minor differences in gene expression are observed between the pMMO-producing cultures. Of these, 87 genes are upregulated during sMMO-producing cells, i.e. during copper-limited growth. Major changes takes place in the respiratory chain between pMMO-and sMMO-producing cells, and quinone are predominantly used as the electron donors for methane oxidation by pMMO. Proposed pathway of methane oxidation in Methylococcus capsulatus cells producing either sMMO or pMMO, overview Methylococcus capsulatus
metabolism the high number of up-regulated genes in cells producing soluble methane monooxygenase shows that Methylococcus capsulatus is highly adapted to copper-limited growth Methylococcus capsulatus