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Literature summary for 1.14.13.248 extracted from

  • Sugai, Y.; Katsuyama, Y.; Ohnishi, Y.
    A nitrous acid biosynthetic pathway for diazo group formation in bacteria (2016), Nat. Chem. Biol., 12, 73-75 .
    View publication on PubMed
Search for more literature for 1.14.13.248

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 NADPH 30°C, pH not specified in the publication Streptomyces cremeus
0.125
-
 L-aspartate 30°C, pH not specified in the publication Streptomyces cremeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2S)-2-nitrosobutanedioate + NADPH + H+ + O2 Streptomyces cremeus
-
 (2S)-2-nitrobutanedioate + NADP+ + H2O
-
 ?
L-aspartate + 3 NADPH + 3 H+ + 3 O2 Streptomyces cremeus
-
 (2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
 ?
L-aspartate + NADPH + H+ + O2 Streptomyces cremeus
-
 N-hydroxy-L-aspartate + NADP+ + H2O
-
 ?
N-hydroxy-L-aspartate + NADPH + H+ + O2 Streptomyces cremeus
-
 N,N-dihydroxy-L-aspartate + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces cremeus A0A0K2JL70
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-nitrosobutanedioate + NADPH + H+ + O2
-
 Streptomyces cremeus (2S)-2-nitrobutanedioate + NADP+ + H2O
-
 ?
L-aspartate + 3 NADPH + 3 H+ + 3 O2
-
 Streptomyces cremeus (2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
 ?
L-aspartate + NADPH + H+ + O2
-
 Streptomyces cremeus N-hydroxy-L-aspartate + NADP+ + H2O
-
 ?
N-hydroxy-L-aspartate + NADPH + H+ + O2
-
 Streptomyces cremeus N,N-dihydroxy-L-aspartate + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
creE
-
 Streptomyces cremeus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14
-
 L-aspartate 30°C, pH not specified in the publication Streptomyces cremeus
1.19
-
 NADPH 30°C, pH not specified in the publication Streptomyces cremeus

General Information

General Information Comment Organism
metabolism CreE catalyzes iterative oxidation of L-aspartic acid to nitrosuccinic acid via N-hydroxyaspartic acid and nitrososuccinic acid. When CreE and CreD are incubated with L-aspartic acid, nitrous acid is the product. CreD accepts nitrosuccinic acid as a substrate only when it is directly delivered by CreE immediately after its synthesis. Both are involved in cremeomycin biosynthesis Streptomyces cremeus