Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.248 extracted from

  • Hagihara, R.; Katsuyama, Y.; Sugai, Y.; Onaka, H.; Ohnishi, Y.
    Novel desferrioxamine derivatives synthesized using the secondary metabolism-specific nitrous acid biosynthetic pathway in Streptomyces davawensis (2018), J. Antibiot., 71, 911-919 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces davaonensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 3 NADPH + 3 H+ + 3 O2 Streptomyces davaonensis
-
(2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
?
L-aspartate + 3 NADPH + 3 H+ + 3 O2 Streptomyces davaonensis DSM 101723
-
(2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces davaonensis K4QXN8
-
-
Streptomyces davaonensis DSM 101723 K4QXN8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, purified by Ni2+ affinity chromatography Streptomyces davaonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 3 NADPH + 3 H+ + 3 O2
-
Streptomyces davaonensis (2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
?
L-aspartate + 3 NADPH + 3 H+ + 3 O2
-
Streptomyces davaonensis DSM 101723 (2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
?

Synonyms

Synonyms Comment Organism
BN159_4422
-
Streptomyces davaonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptomyces davaonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces davaonensis

General Information

General Information Comment Organism
physiological function gene products of BN159_4422 and BN159_4421 synthesizes nitrous acid from L-aspartic acid. Both are involved in the synthesis of desferrioxamine derivatives Streptomyces davaonensis