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Literature summary for 1.14.13.243 extracted from

  • Newman, L.M.; Wackett, L.P.
    Purification and characterization of toluene 2-monooxygenase from Burkholderia cepacia G4 (1995), Biochemistry, 34, 14066-14076 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
2-methylphenol pH 7.5, 23°C Burkholderia cepacia

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the 211 kDa alpha2beta2gamma2 component contains five to six iron atoms Burkholderia cepacia
Iron the 40 kDa subunit contains a [2Fe2S] cluster, and the 211 kDa alpha2beta2gamma2 component contains five to six iron atoms Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methylphenol + NADH + H+ + O2 Burkholderia cepacia
-
3-methylcatechol + NAD+ + H2O
-
?
2-methylphenol + NADH + H+ + O2 Burkholderia cepacia G4
-
3-methylcatechol + NAD+ + H2O
-
?
toluene + NADH + H+ + O2 Burkholderia cepacia
-
2-methylphenol + NAD+ + H2O
-
?
toluene + NADH + H+ + O2 Burkholderia cepacia G4
-
2-methylphenol + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia G4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylphenol + NADH + H+ + O2
-
Burkholderia cepacia 3-methylcatechol + NAD+ + H2O
-
?
2-methylphenol + NADH + H+ + O2
-
Burkholderia cepacia G4 3-methylcatechol + NAD+ + H2O
-
?
toluene + NADH + H+ + O2
-
Burkholderia cepacia 2-methylphenol + NAD+ + H2O
-
?
toluene + NADH + H+ + O2
-
Burkholderia cepacia G4 2-methylphenol + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
More three-component enzyme system, consisting of a 40 kDa polypeptide, a 10.4 kDa polypeptide, and a 211 kDa alpha2beta2gamma2 component, SDS-PAGE Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
2Fe-2S-center
-
Burkholderia cepacia
2Fe-2S-center the 40 kDa subunit contains a [2Fe2S] cluster Burkholderia cepacia
FAD the 40 kDa polypeptide subunit contains one FAD and a [2Fe-2S] cluster Burkholderia cepacia

pI Value

Organism Comment pI Value Maximum pI Value
Burkholderia cepacia 10.4 kDa polypeptide component, isoelectric focussing
-
4.3
Burkholderia cepacia alpha2beta2gamma2 component, isoelectric focussing
-
4.5
Burkholderia cepacia 40 kDa component, isoelectric focussing
-
5.8

General Information

General Information Comment Organism
physiological function enzyme consists of a 40 kDa polypeptide containing one FAD and a [2Fe-2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms. The 40 kDa flavo-iron-sulfur protein oxidizes NADH and transfers electrons to cytochrome c, dyes, and the alpha2beta2gamma2 component, which contains the site for toluene binding and hydroxylation Burkholderia cepacia
physiological function three-component enzyme system, consisting of a 40 kDa polypeptide containing one FAD and a [2Fe2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms. The 40 kDa flavo-iron-sulfur protein oxidizes NADH and transfers electrons to cytochrome c, dyes, and the alpha2beta2gamma2 component. The 10.4 kDa component, added to the other two components and NADH, increases toluene oxidation rates 40 kDa polypeptide containing one FAD and a [2Fe2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms10fold. The alpha2beta2gamma2 component contains the site for toluene binding and hydroxylation Burkholderia cepacia