KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
2-methylphenol | pH 7.5, 23°C | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the 211 kDa alpha2beta2gamma2 component contains five to six iron atoms | Burkholderia cepacia | |
Iron | the 40 kDa subunit contains a [2Fe2S] cluster, and the 211 kDa alpha2beta2gamma2 component contains five to six iron atoms | Burkholderia cepacia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylphenol + NADH + H+ + O2 | Burkholderia cepacia | - |
3-methylcatechol + NAD+ + H2O | - |
? | |
2-methylphenol + NADH + H+ + O2 | Burkholderia cepacia G4 | - |
3-methylcatechol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | Burkholderia cepacia | - |
2-methylphenol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | Burkholderia cepacia G4 | - |
2-methylphenol + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
- |
- |
Burkholderia cepacia G4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methylphenol + NADH + H+ + O2 | - |
Burkholderia cepacia | 3-methylcatechol + NAD+ + H2O | - |
? | |
2-methylphenol + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | 3-methylcatechol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | - |
Burkholderia cepacia | 2-methylphenol + NAD+ + H2O | - |
? | |
toluene + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | 2-methylphenol + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-component enzyme system, consisting of a 40 kDa polypeptide, a 10.4 kDa polypeptide, and a 211 kDa alpha2beta2gamma2 component, SDS-PAGE | Burkholderia cepacia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2Fe-2S-center | - |
Burkholderia cepacia | |
2Fe-2S-center | the 40 kDa subunit contains a [2Fe2S] cluster | Burkholderia cepacia | |
FAD | the 40 kDa polypeptide subunit contains one FAD and a [2Fe-2S] cluster | Burkholderia cepacia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Burkholderia cepacia | 10.4 kDa polypeptide component, isoelectric focussing | - |
4.3 |
Burkholderia cepacia | alpha2beta2gamma2 component, isoelectric focussing | - |
4.5 |
Burkholderia cepacia | 40 kDa component, isoelectric focussing | - |
5.8 |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme consists of a 40 kDa polypeptide containing one FAD and a [2Fe-2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms. The 40 kDa flavo-iron-sulfur protein oxidizes NADH and transfers electrons to cytochrome c, dyes, and the alpha2beta2gamma2 component, which contains the site for toluene binding and hydroxylation | Burkholderia cepacia |
physiological function | three-component enzyme system, consisting of a 40 kDa polypeptide containing one FAD and a [2Fe2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms. The 40 kDa flavo-iron-sulfur protein oxidizes NADH and transfers electrons to cytochrome c, dyes, and the alpha2beta2gamma2 component. The 10.4 kDa component, added to the other two components and NADH, increases toluene oxidation rates 40 kDa polypeptide containing one FAD and a [2Fe2S] cluster, a 10.4 kDa polypeptide that contains no identifiable metals or organic cofactors, and a 211 kDa alpha2beta2gamma2 component containing five to six iron atoms10fold. The alpha2beta2gamma2 component contains the site for toluene binding and hydroxylation | Burkholderia cepacia |