Literature summary for 1.14.13.242 extracted from

Luanloet, T.; Sucharitakul, J.; Chaiyen, P.
Selectivity of substrate binding and ionization of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (2015), FEBS J., 282, 3107-3125 .

Search for more literature for 1.14.13.242

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas sp. MA-1

Protein Variants

Protein Variants	Comment	Organism
Y223E	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1
Y223F	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1
Y223H	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1
Y223T	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1
Y82F	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1
Y82H	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1
Y82R	the mutant shows reduced activity compared to the wild type enzyme	Pseudomonas sp. MA-1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-hydroxy-2-methylpyridine-5-carboxylate + NADPH + H+ + O2	Pseudomonas sp. MA-1	-	2-(acetamidomethylene)succinate + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. MA-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography and G-25 Sephadex gel filtration	Pseudomonas sp. MA-1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxy-2-methylpyridine-5-carboxylate + NADPH + H+ + O2	-	Pseudomonas sp. MA-1	2-(acetamidomethylene)succinate + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
2-methyl-3-hydroxypyridine 5-carboxylic acid oxygenase	-	Pseudomonas sp. MA-1
MHPCO	-	Pseudomonas sp. MA-1

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Pseudomonas sp. MA-1
NADPH	-	Pseudomonas sp. MA-1

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Release 2023.1 (January 2023)