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Literature summary for 1.14.13.22 extracted from
- Cloning and characterization of a cyclohexanone monooxygenase gene from Arthrobacter sp. L661 (2008), Biotechnol. Bioprocess Eng., 13, 40-47.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene chnB, genomic library screening, DNA and amino acid sequence determination and analysis, sequence comparisons, functional expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Arthrobacter sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cyclohexanone + NADPH + H+ + O2 | Arthrobacter sp. | - |
epsilon-caprolactone + NADP+ + H2O | - |
? |  |
| cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. TK6 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. Phi1 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | Arthrobacter sp. L661 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | Arthrobacter sp. BP2 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | Rhodococcus sp. Phi2 | - |
epsilon-caprolactone + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | A5HLX5 | gene chnB | - |
| Arthrobacter sp. | Q84H88 | gene chnB | - |
| Arthrobacter sp. BP2 | Q84H88 | gene chnB | - |
| Arthrobacter sp. L661 | A5HLX5 | gene chnB | - |
| Rhodococcus sp. | Q6RXW1 | gene chnB | - |
| Rhodococcus sp. | Q84H73 | - |
- |
| Rhodococcus sp. | Q84H76 | - |
- |
| Rhodococcus sp. Phi1 | Q84H73 | - |
- |
| Rhodococcus sp. Phi2 | Q84H76 | - |
- |
| Rhodococcus sp. TK6 | Q6RXW1 | gene chnB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme 8.3fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arthrobacter sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the organism is capable of growth on cyclohexanone as a sole carbon source | Arthrobacter sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.75 | - |
purified His-tagged recombinant enzyme | Arthrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-cyclohexandione + NADPH + H+ + O2 | 18% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 2% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 24% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 7% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 24% of the activity with cyclohexanone | Rhodococcus sp. TK6 | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 2% of the activity with cyclohexanone | Rhodococcus sp. Phi1 | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 18% of the activity with cyclohexanone | Arthrobacter sp. L661 | ? + NADP+ + H2O | - |
? | |
| 1,2-cyclohexandione + NADPH + H+ + O2 | 7% of the activity with cyclohexanone | Arthrobacter sp. BP2 | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 11% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 5% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. TK6 | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 5% of the activity with cyclohexanone | Arthrobacter sp. L661 | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 11% of the activity with cyclohexanone | Arthrobacter sp. BP2 | ? + NADP+ + H2O | - |
? | |
| 1,3-cyclohexandione + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. Phi2 | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 112% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 164% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 78% of the activity with cyclohexanone | Arthrobacter sp. | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 90% of the activity with cyclohexanone | Rhodococcus sp. | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 90% of the activity with cyclohexanone | Rhodococcus sp. Phi1 | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 112% of the activity with cyclohexanone | Arthrobacter sp. BP2 | ? + NADP+ + H2O | - |
? | |
| 1,4-cyclohexandione + NADPH + H+ + O2 | 164% of the activity with cyclohexanone | Rhodococcus sp. Phi2 | ? + NADP+ + H2O | - |
? | |
| 2-methyl-cyclohexanone + NADPH + H+ + O2 | 85% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cycloheptane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclohexanone + NADPH + H+ + O2 | best substrate, 130% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cycloheptane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclohexanone + NADPH + H+ + O2 | 159% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclohexanone + NADPH + H+ + O2 | 89% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclohexanone + NADPH + H+ + O2 | best substrate, 118% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclopentanone + NADPH + H+ + O2 | 81% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cyclohexane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclopentanone + NADPH + H+ + O2 | best substrate, 172% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methyl-cyclohexane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclopentanone + NADPH + H+ + O2 | 92% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O | - |
? | |
| 2-methyl-cyclopentanone + NADPH + H+ + O2 | 98% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-3-methylcyclohexane + NADP+ + H2O | - |
? | |
| cyclobutanone + NADPH + H+ + O2 | 3% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| cyclobutanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| cyclobutanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| cyclobutanone + NADPH + H+ + O2 | 92% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| cyclobutanone + NADPH + H+ + O2 | 65% of the activity with cyclohexanone | Rhodococcus sp. | dihydrofuran-2(3H)-one + NADP+ + H2O | - |
? | |
| cyclodecanone + NADPH + H+ + O2 | 2% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| cyclodecanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| cyclodecanone + NADPH + H+ + O2 | 5% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| cyclodecanone + NADPH + H+ + O2 | 6% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cycloundecane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + H+ + O2 | 108% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + H+ + O2 | 166% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + H+ + O2 | 73% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + H+ + O2 | 80% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + H+ + O2 | 90% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Arthrobacter sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. TK6 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. TK6 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. Phi1 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. Phi1 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Arthrobacter sp. L661 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | best substrate, an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. L661 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Arthrobacter sp. BP2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Arthrobacter sp. BP2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | - |
Rhodococcus sp. Phi2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + H+ + O2 | an asymmetric Baeyer-Villiger biooxidation, overview | Rhodococcus sp. Phi2 | epsilon-caprolactone + NADP+ + H2O | - |
? | |
| cyclooctanone + NADPH + H+ + O2 | 17% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclooctanone + NADPH + H+ + O2 | 18% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclooctanone + NADPH + H+ + O2 | 23% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclooctanone + NADPH + H+ + O2 | 4% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclooctanone + NADPH + H+ + O2 | 42% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + H+ + O2 | 37% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + H+ + O2 | 42% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + H+ + O2 | 48% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + H+ + O2 | 65% of the activity with cyclohexanone | Rhodococcus sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + H+ + O2 | 95% of the activity with cyclohexanone | Arthrobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| additional information | no activity with 1,2-cyclohexandione | Rhodococcus sp. | ? | - |
? | |
| additional information | no activity with 1,3-cyclohexandione | Rhodococcus sp. | ? | - |
? | |
| additional information | no activity with cyclodecanone | Rhodococcus sp. | ? | - |
? | |
| additional information | substrate specificity of the recombinant His-tagged enzyme, overview | Arthrobacter sp. | ? | - |
? | |
| additional information | no activity with cyclodecanone | Rhodococcus sp. TK6 | ? | - |
? | |
| additional information | no activity with 1,3-cyclohexandione | Rhodococcus sp. Phi1 | ? | - |
? | |
| additional information | substrate specificity of the recombinant His-tagged enzyme, overview | Arthrobacter sp. L661 | ? | - |
? | |
| additional information | no activity with 1,2-cyclohexandione | Rhodococcus sp. Phi2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHMO | - |
Arthrobacter sp. |
| CHMO | - |
Rhodococcus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
His-tagged recombinant enzyme | Arthrobacter sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | 70% of maximal activity at pH 7.0 within this range, His-tagged recombinant enzyme | Arthrobacter sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | purified recombinant enzyme, 70% remaining activity after 24 h at 4°C | Arthrobacter sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Arthrobacter sp. | |
| NADPH | - |
Rhodococcus sp. | |
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