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Literature summary for 1.14.13.217 extracted from

  • Sanchez, C.; Brana, A.F.; Mendez, C.; Salas, J.A.
    Reevaluation of the violacein biosynthetic pathway and its relationship to indolocarbazole biosynthesis (2006), ChemBioChem, 7, 1231-1240.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NADPH
-
Chromobacterium violaceum

Cloned(Commentary)

Cloned (Comment) Organism
gene vioD, recombinant expression in Escherichia coli strain DH10B and Streptomyces albus strain J1074, coexpression with the other genes of the violacein biosynthesis pathway, reevaluation, overview Chromobacterium violaceum

Protein Variants

Protein Variants Comment Organism
additional information additional coexpression of vioC and/or vioD in Streptomyces albus. Disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors Chromobacterium violaceum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Chromobacterium violaceum VioD is no tryptophan 5-hydroxylase ?
-
?
additional information Chromobacterium violaceum JCM 1249 VioD is no tryptophan 5-hydroxylase ?
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2 Chromobacterium violaceum
-
protoviolaceinate + NAD(P)+
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2 Chromobacterium violaceum JCM 1249
-
protoviolaceinate + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum Q9S3U8 strain JCM1249 or ATCC12472
-
Chromobacterium violaceum JCM 1249 Q9S3U8 strain JCM1249 or ATCC12472
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information VioD is no tryptophan 5-hydroxylase Chromobacterium violaceum ?
-
?
additional information VioD is no tryptophan 5-hydroxylase Chromobacterium violaceum JCM 1249 ?
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
Chromobacterium violaceum protoviolaceinate + NAD(P)+
-
?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
Chromobacterium violaceum JCM 1249 protoviolaceinate + NAD(P)+
-
?

Synonyms

Synonyms Comment Organism
VioD
-
Chromobacterium violaceum
VioD oxygenase
-
Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
NADH
-
Chromobacterium violaceum
NADPH
-
Chromobacterium violaceum

General Information

General Information Comment Organism
evolution VioD belongs to the family of flavin-containing NAD(P)H-dependent monooxygenases Chromobacterium violaceum
malfunction disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors. Gene vioE is essential for violacein biosynthesis. Enzymes VioC and VioD are responsible for the oxygenations at the ox-indole and hydroxyindole, respectively. In the absence of VioC, the expected proviolaceins are accompanied by other compounds: deoxychromoviridans, chromoviridans, and oxychromoviridans Chromobacterium violaceum
metabolism vioD is one gene encoding violacein biosynthesis, VioD appears to act on late steps of violacein biosynthesis, overview Chromobacterium violaceum