Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.208 extracted from

  • Mohamed, M.E.; Zaar, A.; Ebenau-Jehle, C.; Fuchs, G.
    Reinvestigation of a new type of aerobic benzoate metabolism in the proteobacterium Azoarcus evansii (2001), J. Bacteriol., 183, 1899-1908.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
boxA gene Aromatoleum evansii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe the reductase componment BoxA contains 10.4 to 18.4 mol of Fe, and 13.3 to 17.9 mol of acid-labile sulfur per mol of native protein, depending on the method of protein determination Aromatoleum evansii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45883
-
2 * 45883, component BoxA, calculated from sequence Aromatoleum evansii
50000
-
2 * 50000, component BoxA, SDS-PAGE Aromatoleum evansii
98000
-
BoxA, gel filtration Aromatoleum evansii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii second step in aerobic benzoate metabolism, enzyme is induced by benzoate ?
-
?

Organism

Organism UniProt Comment Textmining
Aromatoleum evansii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
BoxA Aromatoleum evansii

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:benzoate-grown cell
-
Aromatoleum evansii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-CoA + NADPH + H+ + O2 second step in aerobic benzoate metabolism, enzyme is induced by benzoate Aromatoleum evansii ?
-
?
additional information purified reductase component BoxA catalyses, in the absence of BoxB, the electron transfer from NADPH to free FAD with a rate of 17 mmol/min*mg protein. This activity is very high compared to the benzoyl-CoA conversion rate with purified BoxAB (0.29 mmol/min*mg BoxA). The reductase component BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA Aromatoleum evansii ?
-
?

Subunits

Subunits Comment Organism
? 2 * 45883, component BoxA, calculated from sequence Aromatoleum evansii
? 2 * 50000, component BoxA, SDS-PAGE Aromatoleum evansii

Synonyms

Synonyms Comment Organism
benzoyl-CoA oxygenase
-
Aromatoleum evansii

Cofactor

Cofactor Comment Organism Structure
FAD the reductase componment BoxA contains 0.72 mol flavin adenine dinucleotide. BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA Aromatoleum evansii
NADPH the reductase component BoxA oxidizes NADPH to transfer electrons, mediated by enzyme-bound FAD, to one or two of the [4Fe-4S] centres of the protein. The flow of electrons from NADPH to the artificial electron acceptor, free FAD, is greatly (10-fold) stimulated by binding of benzoyl-CoA to BoxA. No oxidation of NADH Aromatoleum evansii

pI Value

Organism Comment pI Value Maximum pI Value
Aromatoleum evansii component BoxA, calculated from sequence
-
5.6