Cloned (Comment) | Organism |
---|---|
gene tfdBa, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, expression in Escherichia coli strain BL21, subcloning in Escherichia coli strains JM109 amd T7 Blue | Bradyrhizobium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenol + NADPH + H+ + O2 | Bradyrhizobium sp. | best substrate | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + H+ + O2 | Bradyrhizobium sp. RD5-C2 | best substrate | 3,5-dichlorocatechol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium sp. | A5LGH2 | gene tfdBa | - |
Bradyrhizobium sp. RD5-C2 | A5LGH2 | gene tfdBa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dichlorophenol + NADPH + H+ + O2 | 26% activity compared to 2,4-dichlorophenol as substrate | Bradyrhizobium sp. | ? + NADP+ + H2O | - |
? | |
2,3-dichlorophenol + NADPH + H+ + O2 | 26% activity compared to 2,4-dichlorophenol as substrate | Bradyrhizobium sp. RD5-C2 | ? + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + H+ + O2 | best substrate | Bradyrhizobium sp. | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
2,4-dichlorophenol + NADPH + H+ + O2 | best substrate | Bradyrhizobium sp. RD5-C2 | 3,5-dichlorocatechol + NADP+ + H2O | - |
? | |
3,4-dichlorophenol + NADPH + H+ + O2 | 30% activity compared to 2,4-dichlorophenol as substrate | Bradyrhizobium sp. | ? + NADP+ + H2O | - |
? | |
3,4-dichlorophenol + NADPH + H+ + O2 | 30% activity compared to 2,4-dichlorophenol as substrate | Bradyrhizobium sp. RD5-C2 | ? + NADP+ + H2O | - |
? | |
4-chlorophenol + NADPH + H+ + O2 | 68% activity compared to 2,4-dichlorophenol as substrate | Bradyrhizobium sp. | ? + NADP+ + H2O | - |
? | |
4-chlorophenol + NADPH + H+ + O2 | 68% activity compared to 2,4-dichlorophenol as substrate | Bradyrhizobium sp. RD5-C2 | ? + NADP+ + H2O | - |
? | |
additional information | the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview | Bradyrhizobium sp. | ? | - |
? | |
additional information | the enzyme shows a narrow substrate specificity with chlorophenols, 3,5-dichlorophenol is a poor substrate, overview | Bradyrhizobium sp. RD5-C2 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bradyrhizobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bradyrhizobium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bradyrhizobium sp. |