Cloned (Comment) | Organism |
---|---|
expressed as a fusion to MBP containing an 8His tag at the N-terminus in Escherichia coli BL21T1R cells | Aspergillus fumigatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | substrate inhibition | Aspergillus fumigatus | |
NADP+ | competitive inhibitor with respect to NADPH | Aspergillus fumigatus | |
NADPH | substrate inhibition | Aspergillus fumigatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADPH | pH 7.5, 25°C, oxygen consumption assay | Aspergillus fumigatus | |
0.21 | - |
NADH | pH 7.5, 25°C, oxygen consumption assay | Aspergillus fumigatus | |
0.5 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay | Aspergillus fumigatus | |
0.9 | - |
NADPH | pH 7.5, 25°C, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
0.94 | - |
NADH | pH 7.5, 25°C, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
1.7 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
1.7 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
2 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay | Aspergillus fumigatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
4 * 55000, SDS-PAGE | Aspergillus fumigatus |
57210 | - |
4 * 57210, MALDI-TOF MS | Aspergillus fumigatus |
228000 | - |
gel filtration | Aspergillus fumigatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NAD(P)H + H+ + O2 | Aspergillus fumigatus | the enzyme catalyzes the hydroxylation of L-ornithine in ferrichrome biosynthesis | N5-hydroxy-L-ornithine + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q5SE95 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus fumigatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-ornithine + NAD(P)H + H+ + O2 = N5-hydroxy-L-ornithine + NAD(P)+ + H2O | the enzyme forms a ternary complex with NADP and L-ornithine during catalysis. The enzyme likely proceeds by a sequential kinetic mechanism | Aspergillus fumigatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NAD(P)H + H+ + O2 | the enzyme catalyzes the hydroxylation of L-ornithine in ferrichrome biosynthesis | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD(P)+ + H2O | - |
? | |
L-ornithine + NADH + H+ + O2 | AfSidA is highly specific for its amino acid substrate, only hydroxylating L-ornithine. An 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NAD+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | AfSidA is highly specific for its amino acid substrate, only hydroxylating L-ornithine. An 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH | Aspergillus fumigatus | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 55000, SDS-PAGE | Aspergillus fumigatus |
tetramer | 4 * 57210, MALDI-TOF MS | Aspergillus fumigatus |
Synonyms | Comment | Organism |
---|---|---|
AfSidA | ambiguous | Aspergillus fumigatus |
siderophore A | - |
Aspergillus fumigatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Aspergillus fumigatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
0.5 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
0.63 | - |
NADPH | pH 7.5, 25°C, oxygen consumption assay | Aspergillus fumigatus | |
0.68 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay | Aspergillus fumigatus | |
0.87 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay | Aspergillus fumigatus | |
1.18 | - |
NADH | pH 7.5, 25°C, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
1.25 | - |
NADPH | pH 7.5, 25°C, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
1.36 | - |
NADH | pH 7.5, 25°C, oxygen consumption assay | Aspergillus fumigatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus fumigatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Aspergillus fumigatus | |
NADH | 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH | Aspergillus fumigatus | |
NADPH | 8-fold preference in the catalytic efficiency is determined for NADPH compared to NADH | Aspergillus fumigatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
NADH | pH 7.5, 25°C | Aspergillus fumigatus | |
2.8 | - |
NADPH | pH 7.5, 25°C | Aspergillus fumigatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADH, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
0.29 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADPH, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
0.43 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADH, oxygen consumption assay | Aspergillus fumigatus | |
1.3 | - |
NADPH | pH 7.5, 25°C, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
1.3 | - |
NADH | pH 7.5, 25°C, following the formation of hydroxylated ornithine | Aspergillus fumigatus | |
1.33 | - |
L-ornithine | pH 7.5, 25°C, cosubstrate: NADPH, oxygen consumption assay | Aspergillus fumigatus | |
3.4 | - |
NADH | pH 7.5, 25°C, oxygen consumption assay | Aspergillus fumigatus | |
21.05 | - |
NADPH | pH 7.5, 25°C, oxygen consumption assay | Aspergillus fumigatus |