Cloned (Comment) | Organism |
---|---|
expression as His6-tagged protein in Escherichia coli | Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.286 | - |
L-ornithine | pH 8.0, 25°C | Saccharopolyspora erythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | Saccharopolyspora erythraea | the enzyme is involved in biosynthesis of N5-acetyl-N5-hydroxy-L-ornithine, a building block of the hydroxamate-type siderophore erythrochelin | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + NADPH + H+ + O2 | - |
Saccharopolyspora erythraea | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
L-ornithine + NADPH + H+ + O2 | the enzyme is involved in biosynthesis of N5-acetyl-N5-hydroxy-L-ornithine, a building block of the hydroxamate-type siderophore erythrochelin | Saccharopolyspora erythraea | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
monooxygenase EtcB | - |
Saccharopolyspora erythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharopolyspora erythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.327 | - |
L-ornithine | pH 8.0, 25°C | Saccharopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharopolyspora erythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD-dependent enzyme | Saccharopolyspora erythraea | |
NADPH | no formation of N5-hydroxy-L-ornithine when NADH is utilized instead of NADPH as the reducing cosubstrate | Saccharopolyspora erythraea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
L-ornithine | pH 8.0, 25°C | Saccharopolyspora erythraea |