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Literature summary for 1.14.13.195 extracted from
- Biochemical characterization of a flavin adenine dinculeotide-dependent monooxygenase, ornithine hydroxylase from Pseudomonas aeruginosa, suggests a novel reaction mechanism (2007), Biochemistry, 46, 11930-11937.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | a mixed inhibitor with respect to ornithine | Pseudomonas aeruginosa |  |
| chloride | mixed-type inhibitor with respect to ornithine, but a competitive inhibitor with respect to NADPH | Pseudomonas aeruginosa | |
| L-lysine | mixed-type inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Pseudomonas aeruginosa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pseudomonas aeruginosa | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + NADPH + H+ + O2 | Pseudomonas aeruginosa | - |
L-lysine N6-oxide + NADP+ + H2O | - |
? | |
| L-ornithine + NADPH + H+ + O2 | Pseudomonas aeruginosa | - |
N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| additional information | Pseudomonas aeruginosa | PvdA is the ornithine hydroxylase, which performs the first enzymatic step in preparation of hydroxamate siderophore derivatives, pyoverdin is the hydroxamate siderophore produced by the opportunistic pathogen Pseudomonas aeruginosa under the iron-limiting conditions of the human host, overview. Poor activity with DL-2,3-diaminopropionic acid and D-ornithine, no activity with L-norleucine, 5-aminopentanoic acid, DL-2,4-diaminobutyric acid, and 1,4-diaminobutane, and no activity with NADH as cofactor, substrate specificity, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| flavoprotein | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.321 | - |
L-ornithine hydroxylation | Pseudomonas aeruginosa |
| 0.534 | - |
NADPH oxidation | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | L-lysine N6-oxide + NADP+ + H2O | - |
? | |
| L-ornithine + NADPH + H+ + O2 | - |
Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| L-ornithine + NADPH + H+ + O2 | PvdA is highly specific for both substrate and coenzyme, PvdA/FAD forms a ternary complex with NADPH and ornithine for catalysis | Pseudomonas aeruginosa | N5-hydroxy-L-ornithine + NADP+ + H2O | - |
? | |
| additional information | PvdA is the ornithine hydroxylase, which performs the first enzymatic step in preparation of hydroxamate siderophore derivatives, pyoverdin is the hydroxamate siderophore produced by the opportunistic pathogen Pseudomonas aeruginosa under the iron-limiting conditions of the human host, overview. Poor activity with DL-2,3-diaminopropionic acid and D-ornithine, no activity with L-norleucine, 5-aminopentanoic acid, DL-2,4-diaminobutyric acid, and 1,4-diaminobutane, and no activity with NADH as cofactor, substrate specificity, overview | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | extending the side chain by one methylene group to L-lysine results in significant NADPH oxidation without formation of the hydroxylated product, indicating that the reaction is uncoupled | Pseudomonas aeruginosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ornithine hydroxylase | - |
Pseudomonas aeruginosa |
| PvdA | - |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on | Pseudomonas aeruginosa | |
| additional information | no activity with NADH as cofactor | Pseudomonas aeruginosa | |
| NADPH | dependent on | Pseudomonas aeruginosa | |
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