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Literature summary for 1.14.13.195 extracted from

  • Putignani, L.; Ambrosi, C.; Ascenzi, P.; Visca, P.
    Expression of L-ornithine Ndelta-oxygenase (PvdA) in fluorescent Pseudomonas species: an immunochemical and in silico study (2004), Biochem. Biophys. Res. Commun., 313, 245-257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons Pseudomonas fluorescens
PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons Pseudomonas putida
PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons Burkholderia cepacia
PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons Pseudomonas syringae
PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons Ralstonia solanacearum
PvdA, DNA and amino acid sequence determination and analysis, sequence comparisons, promoter activity analysis during cell growth. Recombinant expression of N-terminally His-tagged full-length and truncated wild-type and mutant enzymes in Escherichia coli strain M15 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of diverse inactive truncation mutants, overview Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ornithine + NADPH + H+ + O2 Pseudomonas fluorescens first step in pyoverdine biosynthesis N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 Pseudomonas putida first step in pyoverdine biosynthesis N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 Burkholderia cepacia first step in pyoverdine biosynthesis N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 Pseudomonas syringae first step in pyoverdine biosynthesis N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 Ralstonia solanacearum first step in pyoverdine biosynthesis N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 Pseudomonas aeruginosa first step in pyoverdine biosynthesis N5-hydroxy-L-ornithine + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
gene pvdA
-
Pseudomonas aeruginosa Q51548 several clinical isolates, gene pvdA
-
Pseudomonas fluorescens
-
strains B10 and PF, gene pvdA
-
Pseudomonas putida
-
strain KT2440, no activity in strain WCS358, gene pvdA
-
Pseudomonas syringae
-
pv. syringae, strain B278a, gene pvdA
-
Ralstonia solanacearum
-
gene pvdA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
Pseudomonas fluorescens
flavoprotein
-
Pseudomonas putida
flavoprotein
-
Burkholderia cepacia
flavoprotein
-
Pseudomonas syringae
flavoprotein
-
Ralstonia solanacearum
flavoprotein
-
Pseudomonas aeruginosa

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged full-length and truncated wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + NADPH + H+ + O2
-
Pseudomonas fluorescens N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2
-
Pseudomonas putida N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2
-
Burkholderia cepacia N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2
-
Pseudomonas syringae N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2
-
Ralstonia solanacearum N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2
-
Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas fluorescens N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas putida N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Burkholderia cepacia N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas syringae N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Ralstonia solanacearum N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
L-ornithine + NADPH + H+ + O2 first step in pyoverdine biosynthesis Pseudomonas aeruginosa N5-hydroxy-L-ornithine + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
More immunochemical peptide mapping, molecular weights calculated for the truncated enzyme fragments, overview Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
L-ornithine-Ndelta-oxygenase
-
Pseudomonas fluorescens
L-ornithine-Ndelta-oxygenase
-
Pseudomonas putida
L-ornithine-Ndelta-oxygenase
-
Burkholderia cepacia
L-ornithine-Ndelta-oxygenase
-
Pseudomonas syringae
L-ornithine-Ndelta-oxygenase
-
Ralstonia solanacearum
L-ornithine-Ndelta-oxygenase
-
Pseudomonas aeruginosa
PvdA
-
Pseudomonas fluorescens
PvdA
-
Pseudomonas putida
PvdA
-
Burkholderia cepacia
PvdA
-
Pseudomonas syringae
PvdA
-
Ralstonia solanacearum
PvdA
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
FAD required, functions between NADPH and enzyme Pseudomonas fluorescens
FAD required, functions between NADPH and enzyme Pseudomonas putida
FAD required, functions between NADPH and enzyme Burkholderia cepacia
FAD required, functions between NADPH and enzyme Pseudomonas syringae
FAD required, functions between NADPH and enzyme Ralstonia solanacearum
FAD required, functions between NADPH and enzyme Pseudomonas aeruginosa
NADPH
-
Pseudomonas fluorescens
NADPH
-
Pseudomonas putida
NADPH
-
Burkholderia cepacia
NADPH
-
Pseudomonas syringae
NADPH
-
Ralstonia solanacearum
NADPH
-
Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa pI values, calculated for the truncated enzyme fragments, overview
-
additional information

Expression

Organism Comment Expression
Pseudomonas fluorescens the enzyme expression is induced by iron up
Pseudomonas putida the enzyme expression is induced by iron up
Burkholderia cepacia the enzyme expression is induced by iron up
Pseudomonas syringae the enzyme expression is induced by iron up
Ralstonia solanacearum the enzyme expression is induced by iron up