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Literature summary for 1.14.13.182 extracted from

  • Schertzer, J.W., Brown, S.A.; Whiteley, M.
    Oxygen levels rapidly modulate Pseudomonas aeruginosa social behaviours via substrate limitation of PqsH (2010), Mol. Microbiol., 77, 1527-1538.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.182

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of recombinant enzyme in Escherichia coli both with and without a N-terminal fusion with the maltose binding protein Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00011
-
 2-heptyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
0.00013
-
 2-nonyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
0.00052
-
 O2 pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
0.0016
-
 2-pentyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
0.014
-
 2-propyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
0.032
-
 NADH pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
0.74
-
 NADPH pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 x * 86000, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-heptyl-4(1H)-quinolone + NADH + H+ + O2 Pseudomonas aeruginosa PqsH catalyzes the terminal step in biosynthesis of the Pseudomonas quinolone signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone. Under anaerobic conditions 2-heptyl-3-hydroxy-4(1H)-quinolone is not produced. The enzyme is a biochemical regulator of Pseudomonas quinolone signal controlled social behaviors in Pseudomonas aeruginosa 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I0Q0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-80°C, purified fusion protein can be stored for one week without loss of enzymatic activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-heptyl-4(1H)-quinolone + NADH + H+ + O2 PqsH catalyzes the terminal step in biosynthesis of the Pseudomonas quinolone signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone. Under anaerobic conditions 2-heptyl-3-hydroxy-4(1H)-quinolone is not produced. The enzyme is a biochemical regulator of Pseudomonas quinolone signal controlled social behaviors in Pseudomonas aeruginosa Pseudomonas aeruginosa 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptyl-4(1H)-quinolone + NADH + H+ + O2 the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons Pseudomonas aeruginosa 2-heptyl-3-hydroxy-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-heptyl-4(1H)-quinolone + NADPH + H+ + O2 kcat/KM for NADH is 20fold higher than kcat/Km for NADPH Pseudomonas aeruginosa 2-heptyl-3-hydroxy-4(1H)-quinolone + NADP+ + H2O
-
 ?
2-nonyl-4(1H)-quinolone + NADH + H+ + O2 the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons Pseudomonas aeruginosa 3-hydroxy-2-nonyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-pentyl-4(1H)-quinolone + NADH + H+ + O2 the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons Pseudomonas aeruginosa 3-hydroxy-2-pentyl-4(1H)-quinolone + NAD+ + H2O
-
 ?
2-propyl-4(1H)-quinolone + NADH + H+ + O2 the alkyl side chain of 2-heptyl-4-quinolone is critical for PqsH activity with the highest activity observed for alkyl chain lengths of 7 and 9 carbons Pseudomonas aeruginosa 3-hydroxy-2-propyl-4(1H)-quinolone + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 86000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
2-heptyl-3,4-dihydroxyquinoline synthase
-
 Pseudomonas aeruginosa
PqsH
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 O2 pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
2.3
-
 2-propyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
2.5
-
 2-nonyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
2.7
-
 2-heptyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
3
-
 2-pentyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
3.2
-
 NADH pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
3.8
-
 NADPH pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NADH kcat/KM for NADH is 20fold higher than kcat/Km for NADPH Pseudomonas aeruginosa
NADPH kcat/KM for NADH is 20fold higher than kcat/Km for NADPH Pseudomonas aeruginosa
riboflavin flavin-dependent monooxygenase, riboflavin is included in the assay Pseudomonas aeruginosa

General Information

General Information Comment Organism
physiological function PqsH catalyzes the terminal step in biosynthesis of the Pseudomonas quinolone signal molecule 2-heptyl-3,4-dihydroxyquinoline. Under anaerobic conditions 2-heptyl-3-hydroxy-4(1H)-quinolone is not produced. The enzyme is a biochemical regulator of Pseudomonas Quinolone Signal controlled social behaviors in Pseudomonas aeruginosa Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1
-
 NADPH pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
17
-
 2-propyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
100
-
 NADH pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
190
-
 2-pentyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
1900
-
 2-nonyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa
2308
-
 O2 pH 8.0, 25°C, cosubstrate: 2-heptyl-4(1H)-quinolone Pseudomonas aeruginosa
2455
-
 2-heptyl-4(1H)-quinolone pH 8.0, 25°C Pseudomonas aeruginosa