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Literature summary for 1.14.13.181 extracted from

  • Dickens, M.L.; Priestley, N.D.; Strohl, W.R.
    In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA (1997), J. Bacteriol., 179, 2641-2650.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
13-deoxycarminomycin + NADPH + H+ + O2 Streptomyces sp. DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin 13-dihydrocarminomycin + NADP+ + H2O
-
?
13-deoxydaunorubicin + NADPH + H+ + O2 Streptomyces sp. DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin 13-dihydrodaunorubicin + NADP+ + H2O
-
?
13-dihydrocarminomycin + NADPH + H+ + O2 Streptomyces sp. DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin carminomycin + NADP+ + H2O
-
?
13-dihydrodaunorubicin + NADPH + H+ + O2 Streptomyces sp. DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin daunorubicin + NADP+ + 2 H2O
-
?
daunomycin + NADPH + H+ + O2 Streptomyces sp. DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and 13-dihydrodaunorubicin and the hydroxylation at C-14 of daunorubicin doxorubicin + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. Q59971
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
13-deoxycarminomycin + NADPH + H+ + O2
-
Streptomyces sp. 13-dihydrocarminomycin + NADP+ + H2O
-
?
13-deoxycarminomycin + NADPH + H+ + O2 DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin Streptomyces sp. 13-dihydrocarminomycin + NADP+ + H2O
-
?
13-deoxydaunorubicin + NADPH + H+ + O2
-
Streptomyces sp. 13-dihydrodaunorubicin + NADP+ + H2O i.e. daunorubicinol ?
13-deoxydaunorubicin + NADPH + H+ + O2 DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin Streptomyces sp. 13-dihydrodaunorubicin + NADP+ + H2O
-
?
13-dihydrocarminomycin + NADPH + H+ + O2
-
Streptomyces sp. carminomycin + NADP+ + H2O
-
?
13-dihydrocarminomycin + NADPH + H+ + O2 DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin Streptomyces sp. carminomycin + NADP+ + H2O
-
?
13-dihydrodaunorubicin + NADPH + H+ + O2 DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and daunorubicinol and the hydroxylation at C-14 of daunorubicin Streptomyces sp. daunorubicin + NADP+ + 2 H2O
-
?
13-dihydrodaunorubicin + NADPH + H+ + O2 i.e. daunorubicinol Streptomyces sp. daunorubicin + NADP+ + 2 H2O
-
?
daunomycin + NADPH + H+ + O2
-
Streptomyces sp. doxorubicin + NADP+ + H2O
-
?
daunomycin + NADPH + H+ + O2 DoxA catalyzes three steps in the daunorubicin/doxorubicin biosynthesis pathway: hydroxylation at C13 of 13-deoxycarminomycin and 13-deoxydaunorubicin, oxidation at C-13 of 13-dihydrocarminomycin and 13-dihydrodaunorubicin and the hydroxylation at C-14 of daunorubicin Streptomyces sp. doxorubicin + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
DoxA
-
Streptomyces sp.

Cofactor

Cofactor Comment Organism Structure
NADPH reduced flavin adenine dinucleotide, reduced flavin mononucleotide, and NADH do not substitute for NADPH in these reactions Streptomyces sp.