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Literature summary for 1.14.13.180 extracted from

  • Filippini, S.; Solinas, M.M.; Breme, U.; Schlüter, M.B.; Gabellini, D.; Biamonti, G.; Colombo, A.L.; Garofano, L.
    Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression (1995), Microbiology, 141, 1007-1016.
    View publication on PubMed
Search for more literature for 1.14.13.180

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of dnrF in Streptomyces liwidans TK23 and in Escherichia coli. Since the fusion protein does not show any hydroxylase activity, the dnrF gene is cloned into the pT7-7 vector that allows the expression of an unfused DnrF protein Streptomyces peucetius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000 Streptomyces peucetius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aklavinone + NADPH + H+ + O2 Streptomyces peucetius
-
 epsilon-rhodomycinone + NADP+ + H2O
-
 ?
aklavinone + NADPH + H+ + O2 Streptomyces peucetius ATCC 29050
-
 epsilon-rhodomycinone + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius P32009
-
-
Streptomyces peucetius ATCC 29050 P32009
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aklavinone + NADPH + H+ + O2
-
 Streptomyces peucetius epsilon-rhodomycinone + NADP+ + H2O
-
 ?
aklavinone + NADPH + H+ + O2 modifications of the aklavinone molecule can influence the efficiency of the hydroxylation carried out by DnrF. While molecules modified in positions C-10 and C-13 are hydroxylated as efficiently as aklavinone (11-deoxycarminomycinone conversion), molecules 14-hydroxylated or 4-methylated are very poor substrates (11-deoxy-4-demethyladriamycinone and 11-deoxydaunomycinone conversion) Streptomyces peucetius epsilon-rhodomycinone + NADP+ + H2O
-
 ?
aklavinone + NADPH + H+ + O2
-
 Streptomyces peucetius ATCC 29050 epsilon-rhodomycinone + NADP+ + H2O
-
 ?
aklavinone + NADPH + H+ + O2 modifications of the aklavinone molecule can influence the efficiency of the hydroxylation carried out by DnrF. While molecules modified in positions C-10 and C-13 are hydroxylated as efficiently as aklavinone (11-deoxycarminomycinone conversion), molecules 14-hydroxylated or 4-methylated are very poor substrates (11-deoxy-4-demethyladriamycinone and 11-deoxydaunomycinone conversion) Streptomyces peucetius ATCC 29050 epsilon-rhodomycinone + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 52000 Streptomyces peucetius

Synonyms

Synonyms Comment Organism
DnrF
-
 Streptomyces peucetius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Streptomyces peucetius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Streptomyces peucetius

Cofactor

Cofactor Comment Organism Structure
NADPH the DnrF enzyme contains two motifs that are common to a number of FAD- and NADPH-dependent enzymes. The enzyme catalyses the aklavinone 11-hydroxylation in a NADPH-dependent reaction Streptomyces peucetius