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Literature summary for 1.14.13.172 extracted from
- Precursor-directed biosynthesis of 5-hydroxytryptophan using metabolically engineered E. coli (2015), ACS Synth. Biol., 4, 554-558 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| enzyme S5H is encoded in the salABCD gene cluster, which locates on the chromosome rather than on a mobile plasmid, functional recombinant expression in Escherichia coli | Cupriavidus necator |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | precursor-directed biosynthesis of 5-hydroxytryptophan using metabolically engineered Escherichia coli and involving the salicylate 5-hydroxylase from Ralstonia eutropha strain H16 | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| salicylate + NADH + H+ + O2 | Cupriavidus necator | - |
2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | Q0KB56 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + NADH + H+ + O2 | - |
Cupriavidus necator | 5-hydroxyanthranilate + NAD+ + H2O | - |
? | |
| additional information | 2-aminobenzoate might be a substrate for the enzyme | Cupriavidus necator | ? | - |
? | |
| salicylate + NADH + H+ + O2 | - |
Cupriavidus necator | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nagG | - |
Cupriavidus necator |
| nagG | oxygenase component | Cupriavidus necator |
| S5H | - |
Cupriavidus necator |
| SalABCD | - |
Cupriavidus necator |
| SALD | enzyme complex component | Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | SalABCD requires NAD(P)H as the cofactor instead of BH4 | Cupriavidus necator | |
| NADH | - |
Cupriavidus necator | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme S5H hydroxylates salicylate into gentisate and is involved in the degradation of aromatic compounds. SalD is an essential component of the S5H complex | Cupriavidus necator |
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Release 2023.1 (January 2023)
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