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Literature summary for 1.14.13.163 extracted from

  • Yu, H.; Tang, H.; Zhu, X.; Li, Y.; Xu, P.
    Molecular mechanism of nicotine degradation by a newly isolated strain, Ochrobactrum sp. strain SJY1 (2015), Appl. Environ. Microbiol., 81, 272-281 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.163

Cloned(Commentary)

Cloned (Comment) Organism
gene vppD cloned from a 97-kbp DNA fragment containing six nicotine degradation-related genes which is obtained by gap closing from the genome sequence of strain SJY1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Ochrobactrum sp.

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ strong inhibition Ochrobactrum sp.
Cu2+ strong inhibition Ochrobactrum sp.
Zn2+ strong inhibition Ochrobactrum sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.112
-
 NADH pH 8.0, 25°C, recombinant His-tagged enzyme Ochrobactrum sp.
0.201
-
 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate pH 8.0, 25°C, recombinant His-tagged enzyme Ochrobactrum sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 Ochrobactrum sp.
-
 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Ochrobactrum sp. A0A075XAG5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. FAD is partly lost during the purification Ochrobactrum sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:nicotinic acid-grown cell strain SJY uses nicotine as a sole source of carbon, nitrogen, and energy Ochrobactrum sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
 Ochrobactrum sp. 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
VppD
-
 Ochrobactrum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Ochrobactrum sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.5
-
 NADH pH 8.0, 25°C, recombinant His-tagged enzyme Ochrobactrum sp.
21.6
-
 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate pH 8.0, 25°C, recombinant His-tagged enzyme Ochrobactrum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Ochrobactrum sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Ochrobactrum sp.
NADH
-
 Ochrobactrum sp.

General Information

General Information Comment Organism
evolution sequence alignment and phylogenetic analysis suggests that the VPP pathway, which evolved independently from nicotinic acid degradation, might have a closer relationship with the pyrrolidine pathway Ochrobactrum sp.
metabolism strain SJY1 efficiently degrades nicotine via a variant of the pyridine and pyrrolidine pathways (the VPP pathway), highlighting bacterial metabolic diversity in relation to nicotine degradation, a 97-kbp DNA fragment containing six nicotine degradation-related genes is obtained by gap closing from the genome sequence of strain SJY1, gene vppD gene encodes an NADH-dependent flavin-containing monooxygenase, which catalyzes the hydroxylation of 6-hydroxy-3-succinoylpyridine to 2,5-dihydroxypyridine. Nicotine degradation pathway in strain SJY1, detailed overview Ochrobactrum sp.
additional information the ativity of VppD is 10fold higher than the activity of the hydroxylase (HspB) from Pseudomonas putida strain S16 Ochrobactrum sp.