Cloned (Comment) | Organism |
---|---|
gene pgl, recombinant expression of truncated protein OsCAO1pgl fused with GFP (35S::OsCAO1pgl-GFP) and transformed into Nicotiana benthamiana via Agrobacterium sp., specific expression in chloroplasts although the truncated enzyme lacks the signal sequence | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of the truncated enzyme mutant OsCAO1pgl. The premature translational termination of OsCAO1 in mutant pgl does not affect OsCAO1 localization, the mutation nevertheless results in the pale-green phenotype. Expression levels of Chl synthesis-associated genes in mutant pgl, and phenotype, overview | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | the chloroplast localization signal of OsCAO1 likely exists in the N-terminus | Oryza sativa Japonica Group | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ | Oryza sativa Japonica Group | - |
chlorophyllide b + 3 H2O + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q8S7E1 | CAO1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
chlorenchyma | PGL, encoding chlorophyllide a oxygenase 1, is mainly expressed in the chlorenchyma | Oryza sativa Japonica Group | - |
culm | - |
Oryza sativa Japonica Group | - |
leaf blade | - |
Oryza sativa Japonica Group | - |
leaf sheath | - |
Oryza sativa Japonica Group | - |
panicle | - |
Oryza sativa Japonica Group | - |
root | very low enzyme content | Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ | - |
Oryza sativa Japonica Group | chlorophyllide b + 3 H2O + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAO | - |
Oryza sativa Japonica Group |
chlorophyllide a oxygenase 1 | - |
Oryza sativa Japonica Group |
OsCAO1 | - |
Oryza sativa Japonica Group |
PGL | - |
Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa Japonica Group |
Organism | Comment | Expression |
---|---|---|
Oryza sativa Japonica Group | PGL, encoding chlorophyllide a oxygenase 1, is induced in the light-dependent chlorophyll synthesis process | up |
General Information | Comment | Organism |
---|---|---|
malfunction | rice mutant pale green leaf (pgl) exhibits a lower chlorophyll content with a reduced and disorderly thylakoid ultrastructure, which decreases the photosynthesis rate and results in reduced grain yield and quality compared to the wild-type. In addition, mutant pgl exhibits premature senescence in both natural and dark-induced conditions and more severe chlorophyll degradation and reactive oxygen species accumulation than does the wild-type. Mutant pgl is sensitive to heat stress. The pgl plants exhibit a pale-green leaf in the paddy field and present a severe withered phenotype in the leaf tip | Oryza sativa Japonica Group |
physiological function | PGL, encoding chlorophyllide a oxygenase 1, impacts leaf senescence and indirectly affects grain yield and quality in rice. The enzyme is required for the light-dependent Chl synthesis during greening of etiolated plants. PGL might also affects the ROS scavenging system under low light | Oryza sativa Japonica Group |