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Literature summary for 1.14.13.113 extracted from

  • O'Leary, S.E.; Hicks, K.A.; Ealick, S.E.; Begley, T.P.
    Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase (2009), Biochemistry, 48, 3033-3035.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.113

Cloned(Commentary)

Cloned (Comment) Organism
cloning and heterologous overexpression as N-terminally six-His tagged protein Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 Urate pH 8.0, cosubstrate 1.5 mM NADH Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 recombinant enzyme including the affinity tag, determined by SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella pneumoniae urate oxidase can catalyze the slow conversion of NADPH to NADP+ in the absence of urate ?
-
 ?
urate + NADH + H+ + O2 Klebsiella pneumoniae part of the purine catabolic pathway 5-hydroxyisourate + NAD+ + H2O unstable in aqueous buffer, hydrolyzing spontaneously to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme is purified by Ni2+-nitrilotriacetic acid chromatography Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information urate oxidase can catalyze the slow conversion of NADPH to NADP+ in the absence of urate Klebsiella pneumoniae ?
-
 ?
urate + NADH + H+ + O2 part of the purine catabolic pathway Klebsiella pneumoniae 5-hydroxyisourate + NAD+ + H2O unstable in aqueous buffer, hydrolyzing spontaneously to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline ?
urate + NADH + H+ + O2 urate hydroxylase shows selectivity (V/K ratio of 10) for NADH over NADPH Klebsiella pneumoniae 5-hydroxyisourate + NAD+ + H2O unstable in aqueous buffer, hydrolyzing spontaneously to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline ?

Synonyms

Synonyms Comment Organism
FAD-dependent urate oxidase
-
 Klebsiella pneumoniae
HpxO
-
 Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42
-
 Urate pH 8.0, 1.5 mM NADH as cosubstrate Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme is a flavoprotein Klebsiella pneumoniae
NADH the enzyme shows selectivity (V/K ratio of 10) for NADH over NADPH Klebsiella pneumoniae
NADPH the enzyme shows selectivity (V/K ratio of 10) for NADH over NADPH Klebsiella pneumoniae