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Literature summary for 1.14.13.111 extracted from

  • Reichenbecher, W.; Murrell, J.C.
    Purification and partial characterization of the hydroxylase component of the methanesulfonic acid mono-oxygenase from Methylosulfonomonas methylovora strain M2 (2000), Eur. J. Biochem., 267, 4763-4769.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning of the reductase encoded by the msmD gene. Cloning and overexpression of the msmD gene, encoding the reductase component, as a GST fusion protein results in the expression of a 65000 Da polypeptide matching the size of the GST protein plus the reductase protein when induced with isopropyl thio-beta-D-galactoside. The fusion between the two proteins is unstable, and purification by affinity chromatography is not possible Methylosulfonomonas methylovora

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Methylosulfonomonas methylovora 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron presence of iron-sulfur centres. The two-component hydroxylase of methanesulfonic acid monooxygenase preparations containes 1 mol sulfide and 3 mol iron per mol alphabeta-monomer Methylosulfonomonas methylovora
additional information chromium, cobalt, copper, lead, nickel, molybdenum, tungsten and vanadium are not detected in two-component hydroxylase of methanesulfonic acid monooxygenase Methylosulfonomonas methylovora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
3 * 43700 (MsmA) + 3 * 23000 (MsmB), two-component hydroxylase of methanesulfonic acid monooxygenase, SDS-PAGE Methylosulfonomonas methylovora
43700
-
3 * 43700 (MsmA) + 3 * 23000 (MsmB), two-component hydroxylase of methanesulfonic acid monooxygenase, SDS-PAGE Methylosulfonomonas methylovora
209000
-
two-component hydroxylase of methanesulfonic acid monooxygenase, gel filtration Methylosulfonomonas methylovora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CH3SO3- + O2 + NADH + H+ Methylosulfonomonas methylovora MSAMO is specifically induced during growth on methanesulfonate formaldehyde + HSO3- + NAD+ + H2O
-
?
CH3SO3- + O2 + NADH + H+ Methylosulfonomonas methylovora M2 MSAMO is specifically induced during growth on methanesulfonate formaldehyde + HSO3- + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Methylosulfonomonas methylovora
-
-
-
Methylosulfonomonas methylovora M2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of two-component hydroxylase of methanesulfonic acid monooxygenase. Purification of the reductase component (MsmD) using a range of chromatographic techniques fails Methylosulfonomonas methylovora

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.88
-
-
Methylosulfonomonas methylovora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH3SO3- + O2 + NADH + H+
-
Methylosulfonomonas methylovora formaldehyde + HSO3- + NAD+ + H2O
-
?
CH3SO3- + O2 + NADH + H+ MSAMO is specifically induced during growth on methanesulfonate Methylosulfonomonas methylovora formaldehyde + HSO3- + NAD+ + H2O
-
?
CH3SO3- + O2 + NADH + H+
-
Methylosulfonomonas methylovora M2 formaldehyde + HSO3- + NAD+ + H2O
-
?
CH3SO3- + O2 + NADH + H+ MSAMO is specifically induced during growth on methanesulfonate Methylosulfonomonas methylovora M2 formaldehyde + HSO3- + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
hexamer 3 * 43700 (MsmA) + 3 * 23000 (MsmB), two-component hydroxylase of methanesulfonic acid monooxygenase, SDS-PAGE Methylosulfonomonas methylovora

Synonyms

Synonyms Comment Organism
methanesulfonic acid monooxygenase
-
Methylosulfonomonas methylovora
MSAMO
-
Methylosulfonomonas methylovora

Cofactor

Cofactor Comment Organism Structure
additional information flavin is absent in the two-component hydroxylase of methanesulfonic acid monooxygenase Methylosulfonomonas methylovora
NADH
-
Methylosulfonomonas methylovora

pI Value

Organism Comment pI Value Maximum pI Value
Methylosulfonomonas methylovora two-component hydroxylase of methanesulfonic acid monooxygenase, chromatofocusing
-
4.9