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Literature summary for 1.14.13.1 extracted from

  • Puntus, I.; Vlasova, E.; Sokolov, A.; Zakharchenko, N.; Funtikova, T.
    Properties of non-homologous salicylate hydroxylases of Pseudomonas bacteria (2015), Appl. Biochem. Microbiol., 51, 215-221 .
    View publication on PubMed
Search for more literature for 1.14.13.1

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by FMN and riboflavin Pseudomonas fluorescens
additional information not activated by FMN and riboflavin Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 salicylate at pH 7.5, temperature not specified in the publication Pseudomonas fluorescens
0.0031
-
 salicylate at pH 7.5, temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
salicylate + NADH + H+ + O2 Pseudomonas fluorescens
-
 catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 Pseudomonas putida
-
 catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 Pseudomonas fluorescens 142 NF
-
 catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 Pseudomonas putida BS3701
-
 catechol + NAD+ + H2O + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens 142 NF
-
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida BS3701
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration Pseudomonas fluorescens
DEAE-Toyopearl 650M column chromatography, phenyl Sepharose CL6B column chromatography, and Sephacryl gel filtration Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.63
-
 after 45fold purification, at pH 7.5, temperature not specified in the publication Pseudomonas putida
7.55
-
 after 69fold purification, at pH 7.5, temperature not specified in the publication Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxy-2-naphthoate + NADH + H+ + O2 25% activity compared to salicylate Pseudomonas fluorescens ?
-
 ?
1-hydroxy-2-naphthoate + NADH + H+ + O2 83% activity compared to salicylate Pseudomonas putida ?
-
 ?
1-hydroxy-2-naphthoate + NADH + H+ + O2 25% activity compared to salicylate Pseudomonas fluorescens 142 NF ?
-
 ?
1-hydroxy-2-naphthoate + NADH + H+ + O2 83% activity compared to salicylate Pseudomonas putida BS3701 ?
-
 ?
3-aminosalicylate + NADH + H+ + O2 11% activity compared to salicylate Pseudomonas fluorescens 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-aminosalicylate + NADH + H+ + O2 62% activity compared to salicylate Pseudomonas putida 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-aminosalicylate + NADH + H+ + O2 11% activity compared to salicylate Pseudomonas fluorescens 142 NF 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-aminosalicylate + NADH + H+ + O2 62% activity compared to salicylate Pseudomonas putida BS3701 3-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-chlorosalicylate + NADH + H+ + O2 15% activity compared to salicylate Pseudomonas fluorescens 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-chlorosalicylate + NADH + H+ + O2 23% activity compared to salicylate Pseudomonas putida 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-chlorosalicylate + NADH + H+ + O2 15% activity compared to salicylate Pseudomonas fluorescens 142 NF 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-chlorosalicylate + NADH + H+ + O2 23% activity compared to salicylate Pseudomonas putida BS3701 3-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-methylsalicylate + NADH + H+ + O2 47% activity compared to salicylate Pseudomonas fluorescens 3-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
3-methylsalicylate + NADH + H+ + O2 86% activity compared to salicylate Pseudomonas putida 3-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
4-aminosalicylate + NADH + H+ + O2 130% activity compared to salicylate Pseudomonas putida 4-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
4-aminosalicylate + NADH + H+ + O2 94% activity compared to salicylate Pseudomonas fluorescens 4-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
4-chlorosalicylate + NADH + H+ + O2 43% activity compared to salicylate Pseudomonas fluorescens 4-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
4-chlorosalicylate + NADH + H+ + O2 78% activity compared to salicylate Pseudomonas putida 4-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
4-methylsalicylate + NADH + H+ + O2 164% activity compared to salicylate Pseudomonas putida 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
4-methylsalicylate + NADH + H+ + O2 166% activity compared to salicylate Pseudomonas fluorescens 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
5-aminosalicylate + NADH + H+ + O2 50% activity compared to salicylate Pseudomonas putida 5-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
5-aminosalicylate + NADH + H+ + O2 80% activity compared to salicylate Pseudomonas fluorescens 5-aminobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
5-chlorosalicylate + NADH + H+ + O2 14% activity compared to salicylate Pseudomonas fluorescens 5-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
5-chlorosalicylate + NADH + H+ + O2 18% activity compared to salicylate Pseudomonas putida 5-chlorobenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
5-hydroxysalicylate + NADH + H+ + O2 132% activity compared to salicylate Pseudomonas fluorescens 2,5-dihydroxyphenol + NAD+ + H2O + CO2
-
 ?
5-hydroxysalicylate + NADH + H+ + O2 84% activity compared to salicylate Pseudomonas putida 2,5-dihydroxyphenol + NAD+ + H2O + CO2
-
 ?
5-methylsalicylate + NADH + H+ + O2 40% activity compared to salicylate Pseudomonas putida 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
5-methylsalicylate + NADH + H+ + O2 81% activity compared to salicylate Pseudomonas fluorescens 4-methylbenzene-1,2-diol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas fluorescens catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 100% activity Pseudomonas fluorescens catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 100% activity Pseudomonas putida catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas fluorescens 142 NF catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 100% activity Pseudomonas fluorescens 142 NF catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2
-
 Pseudomonas putida BS3701 catechol + NAD+ + H2O + CO2
-
 ?
salicylate + NADH + H+ + O2 100% activity Pseudomonas putida BS3701 catechol + NAD+ + H2O + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, SDS-PAGE Pseudomonas fluorescens
monomer 1 * 46000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
NahG
-
 Pseudomonas fluorescens
NahG
-
 Pseudomonas putida
NahU
-
 Pseudomonas fluorescens
NahU
-
 Pseudomonas putida
salicylate hydroxylase
-
 Pseudomonas fluorescens
salicylate hydroxylase
-
 Pseudomonas putida
salicylate, NADH: oxygen oxidoreductase
-
 Pseudomonas fluorescens
salicylate, NADH: oxygen oxidoreductase
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas fluorescens
FAD
-
 Pseudomonas putida
NADH
-
 Pseudomonas fluorescens
NADH
-
 Pseudomonas putida