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Literature summary for 1.14.12.24 extracted from

  • Lin, J.M.; Stark, B.C.; Webster, D.A.
    Effects of Vitreoscilla hemoglobin on the 2,4-dinitrotoluene (2,4-DNT) dioxygenase activity of Burkholderia and on 2,4-DNT degradation in two-phase bioreactors (2003), J. Ind. Microbiol. Biotechnol., 30, 362-368.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Vitreoscilla hemoglobin 74% increase of activity at 0.04 mM Burkholderia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
2,4-dinitrotoluene at pH 7.2 and 37°C Burkholderia sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Burkholderia sp. 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
171000
-
calculated from amino acid sequence Burkholderia sp.
187000
-
gel filtration Burkholderia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dinitrotoluene + NADH + H+ + O2 Burkholderia sp.
-
4-methyl-5-nitrocatechol + nitrite + NAD+
-
?
2,4-dinitrotoluene + NADH + H+ + O2 Burkholderia sp. DNT
-
4-methyl-5-nitrocatechol + nitrite + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
-
-
Burkholderia sp. DNT
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE cellulose column chromatography and Sephadex G150 gel filtration Burkholderia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19
-
crude enzyme, at pH 7.2 and 37°C Burkholderia sp.
385
-
after 20.3fold purification, at pH 7.2 and 37°C Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrotoluene + NADH + H+ + O2
-
Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
?
2,4-dinitrotoluene + NADH + H+ + O2
-
Burkholderia sp. DNT 4-methyl-5-nitrocatechol + nitrite + NAD+
-
?

Synonyms

Synonyms Comment Organism
2,4-DNT dioxygenase
-
Burkholderia sp.