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Literature summary for 1.14.12.18 extracted from

  • Ohmori, T.; Morita, H.; Tanaka, M.; Miyauchi, K.; Kasai, D.; Furukawa, K.; Miyashita, K.; Ogawa, N.; Masai, E.; Fukuda, M.
    Development of a strain for efficient degradation of polychlorinated biphenyls by patchwork assembly of degradation pathways (2011), J. Biosci. Bioeng., 111, 437-442.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes bphAaAbAcAd, encoding the large and small subunits of the terminal oxygenase component and the ferredoxin and reductase subunits responsible for electron transfer from NADH to the large subunit. Functional expression Burkholderia sp. strain NK8 is only successful by combining the bphAaAbAcAd genes of RHA1 and bphA3A4 of Pseudomonas pseudoalcaligenes KF707, introduction of a plasmid containing the RHA1 bphAaAb and KF707 bphA3A4 genes plus the RHA1 bphB2C1D1 genes into strain NK8. The remaining enzyme genes involved in the transformation of biphenyl to benzoate, bphB2C1D1, which encodes dehydrogenase, ring-cleavage dioxygenase and hydrolase, confer activities to NK8, installation of the polychlorinated biphenyls degradation pathway, overview Rhodococcus jostii

Protein Variants

Protein Variants Comment Organism
additional information construction of sets of BphA genes by combining the bphAaAbAcAd genes of RHA1 and bphA3A4 of Pseudomonas pseudoalcaligenes KF707, encoding the ferredoxin and reductase subunits, to obtain the BphA activity of RHA1 in chlorobenzoate-degrading Burkholderia sp. strain NK8, which has an insertion in the cbeA gene to inactivate CBA dioxygenase. Hybrid derivatives of BphA containing the KF707 bphA3 confer BphA activity to NK8, and a derivative containing the RHA1 bphAaAb and KF707 bphA3A4 genes exhibit the highest BphA activity. A plasmid containing the RHA1 bphAaAb and KF707 bphA3A4 genes plus the RHA1 bphB2C1D1 genes is constructed and introduced into strain NK8 Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biphenyl + NADH + H+ + O2 Rhodococcus jostii
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(1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biphenyl + NADH + H+ + O2
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Rhodococcus jostii (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+
-
?

Synonyms

Synonyms Comment Organism
BphA
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Rhodococcus jostii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodococcus jostii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rhodococcus jostii

General Information

General Information Comment Organism
additional information the multicomponent enzyme consists of small and large subunits of the oxygenase component, and ferredoxin and reductase components forming a redox chain in the overall reaction, overview Rhodococcus jostii