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Literature summary for 1.14.12.15 extracted from

  • Shigematsu, T.; Yumihara, K.; Ueda, Y.; Morimura, S.; Kida, K.
    Purification and gene cloning of the oxygenase component of the terephthalate 1,2-dioxygenase system from Delftia tsuruhatensis strain T7 (2003), FEMS Microbiol. Lett., 220, 255-260.
    View publication on PubMed
Search for more literature for 1.14.12.15

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Delftia tsuruhatensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE Delftia tsuruhatensis
46000
-
 alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE Delftia tsuruhatensis
235000
-
 gel filtration Delftia tsuruhatensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
terephthalate + NADH + O2 + H+ Delftia tsuruhatensis
-
 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?
terephthalate + NADH + O2 + H+ Delftia tsuruhatensis T7
-
 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Delftia tsuruhatensis Q8GRC1
-
-
Delftia tsuruhatensis T7 Q8GRC1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Delftia tsuruhatensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
 purified enzyme Delftia tsuruhatensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
terephthalate + NADH + O2 + H+
-
 Delftia tsuruhatensis (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?
terephthalate + NADH + O2 + H+
-
 Delftia tsuruhatensis T7 (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate + NAD+
-
 ?

Subunits

Subunits Comment Organism
hexamer alpha-beta, 3 * 46000 + 3 * 17000, SDS-PAGE Delftia tsuruhatensis

Cofactor

Cofactor Comment Organism Structure
iron-sulfur centre [2Fe-2S] Rieske center Delftia tsuruhatensis
NADH
-
 Delftia tsuruhatensis

pI Value

Organism Comment pI Value Maximum pI Value
Delftia tsuruhatensis isoelectric focusing, beta subunit
-
 4.9
Delftia tsuruhatensis isoelectric focusing, alpha subunit
-
 5.3