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Literature summary for 1.14.12.10 extracted from

  • Yamaguchi, M.; Fujisawa, H.
    Subunit structure of oxygenase component in benzoate-1,2-dioxygenase system from Pseudomonas arvilla C-1 (1982), J. Biol. Chem., 257, 12497-12502.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron sulfur protein with [2Fe-2S]clusters and additional iron atoms Pseudomonas putida
Iron alpha-subunit contains 8.2 mol of iron Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE Pseudomonas putida
38000
-
1 * 38000, reductase component of the enzyme system Pseudomonas putida
50000
-
alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE Pseudomonas putida
201000
-
sedimentation equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzoate + O2 + NADH + H+ Pseudomonas putida
-
2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH + H+ Pseudomonas putida C-1
-
2-hydro-1,2-dihydroxybenzoate + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
C-1
-
Pseudomonas putida C-1
-
C-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoate + O2 + NADH + H+
-
Pseudomonas putida 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?
benzoate + O2 + NADH + H+
-
Pseudomonas putida C-1 2-hydro-1,2-dihydroxybenzoate + NAD+
-
?

Subunits

Subunits Comment Organism
hexamer alpha3,beta3, 3 * 20000 + 3 * 50000, oxygenase component of the enzyme system, SDS-PAGE Pseudomonas putida
monomer 1 * 38000, reductase component of the enzyme system Pseudomonas putida