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Literature summary for 1.14.11.8 extracted from

  • Vaz, F.M.; Ofman, R.; Westinga, K.; Back, J.W.; Wanders, R.J.A.
    Molecular and biochemical characterization of rat epsilon-N-trimethyllysine hydroxylase, the first enzyme of carnitine biosynthesis (2001), J. Biol. Chem., 276, 33512-33517.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert Homo sapiens
when the putative cDNA is expressed in either Escherichia coli or Saccharomyces cerevisiae no enzyme activity can be detected in lysates of theses cells. High activity can be measured in lysates of COS cells transfected with the putative rat cDNA, whereas only low activity is measured in lysates of cells transfected with the pcDNA3 vector without insert Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.109
-
2-oxoglutarate
-
Rattus norvegicus
1.1
-
N6,N6,N6-Trimethyl-L-lysine pH 6.7, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion exclusively in Rattus norvegicus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Km: 0.054 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
2 * 43000, calculation from nucleotide sequence Rattus norvegicus
87000
-
gel filtration, non-denaturing PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 Rattus norvegicus first enzyme of the L-carnitine biosynthesis 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NVH6
-
-
Mus musculus Q91ZE0
-
-
Rattus norvegicus Q91ZW6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is synthesized as a 47500 Da precursor and processed to a mature protein of 43000 Da, presumably upon import into mitochondria Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0586
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
Homo sapiens 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
?
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2
-
Rattus norvegicus 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
?
N6,N6,N6-trimethyl-L-lysine + 2-oxoglutarate + O2 first enzyme of the L-carnitine biosynthesis Rattus norvegicus 3-hydroxy-N6,N6,N6-trimethyl-L-lysine + succinate + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 43000, calculation from nucleotide sequence Rattus norvegicus

Synonyms

Synonyms Comment Organism
epsilon-N-trimethyllysine hydroxylase
-
Homo sapiens
epsilon-N-trimethyllysine hydroxylase
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 at 37°C Rattus norvegicus