Cloned (Comment) | Organism |
---|---|
gene CG15835 or Kdm4A, sequence comparisons, recombinant overexpression of FLAG-tagged wild-type and mutant enzymes in S2 cells | Drosophila melanogaster |
gene CG33182, sequence comparisons, recombinant overexpression of FLAG-tagged enzyme in S2 cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
H195A | site-directed mutagenesis, mutation of an Fe2+ binding residue, abolishes demethylase activity of dJMJD2(1)/CG15835 | Drosophila melanogaster |
additional information | construction of transgenic lines carrying a UASGAL4-CG15835-Flag construct, where expression of dJMJD2(1)/CG15835 is under the control of the yeast activator GAL4, allowing its overexpression upon crossing with lines expressing GAL4. Overexpression of CG15835 results in spreading of HP1 into euchromatin and a strong decrease on the levels of H3K9me3 and H3K36me3 | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for catalytic activity | Drosophila melanogaster | |
Fe2+ | required for catalytic activity. The coordination of Fe(II) involves residues H195, E197 and H223 | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 | Drosophila melanogaster | - |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 | Drosophila melanogaster | - |
[histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9V333 | - |
- |
Drosophila melanogaster | Q9V6L0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Drosophila melanogaster | - |
salivary gland | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | dJMJD2(1)/CG15835 is capable of demethylating H3K9me3 and H3K36me3 | Drosophila melanogaster | ? | - |
? | |
additional information | dJMJD2(2)/CG33182 is capable of demethylating H3K9me3 and H3K36me3 | Drosophila melanogaster | ? | - |
? | |
[histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 | - |
Drosophila melanogaster | [histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 | - |
Drosophila melanogaster | [histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CG15835 | - |
Drosophila melanogaster |
CG33182 | - |
Drosophila melanogaster |
KDM4A | - |
Drosophila melanogaster |
KDM4B | - |
Drosophila melanogaster |
LD33386 | - |
Drosophila melanogaster |
More | see also EC 1.14.11.66 | Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme CG15835 shows higher identity to mammalian JMJD2D (40%) than to any of the other mammalian JMJD2 isoforms (22%) | Drosophila melanogaster |
evolution | enzyme CG33182 shows higher identity to mammalian JMJD2D (40%) than to any of the other mammalian JMJD2 isoforms (22%) | Drosophila melanogaster |
malfunction | overexpression of CG15835 results in spreading of HP1 into euchromatin and a strong decrease on the levels of H3K9me3 and H3K36me3, while the levels of H3K4me3 and H3K27me3 are not significantly altered. Demethylase activity of dJMJD2(1)/CG15835 depends on the JmjC domain, as it is abolished by mutations that affect its catalytic activity. The single-point mutation H195A, mutating one of the Fe2+ binding residues, abolishes demethylase activity of dJMJD2(1)/CG15835 | Drosophila melanogaster |
physiological function | H3K36me3 acts as a barrier that prevents spreading of HP1 into euchromatin. Both H3K36 and H3K4 methylation associate to actively transcribed genes, suggesting that gene activity is a main determinant to delimit hetero- and euchromatic territories | Drosophila melanogaster |
physiological function | H3K36me3 acts as a barrier that prevents spreading of HP1 into euchromatin. Both H3K36 and H3K4 methylation associate to actively transcribed genes, suggesting that gene activity is a main determinant to delimit hetero- and euchromatic territories. dJMJD2(1)/CG15835 influences heterochromatin organization, but is excluded from heterochromatin. Overexpression of dJMJD2(1)/CG15835 does not affect the pattern of H3K9me2,3 at heterochromatin. dJMJD2(1)/CG15835 localizes to multiple euchromatic sites, where it mostly regulates H3K36me3, as its overexpression results in a strong decrease in the levels of H3K36me3. dJMJD2(1)/CG15835 regulates spreading of HP1 | Drosophila melanogaster |