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Literature summary for 1.14.11.6 extracted from
- Thymine 7-hydroxylase from Neurospora crassa. Substrate specificity studies (1977), Biochim. Biophys. Acta, 481, 431-437.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethylthymine | 70% inhibition at 5 mM with thymine as substrate | Neurospora crassa |  |
| 1-ethyluracil | 60% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 1-methylthymine | 73% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 1-Methyluracil | 80% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 2,4-dimethoxy-5-methylpyrimidine | 68% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5,6-Dihydrothymine | 73% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5-Aminouracil | 100% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5-Bromouracil | 95% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5-Formyluracil | 79% inhibition at 2.5 mM with thymine as substrate | Neurospora crassa | |
| 5-Hydroxyuracil | 92% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5-Mercaptouracil | 60% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5-methylaminouracil | 100% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 5-Nitrouracil | 70% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 6-Azathymine | 94% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 6-methylthymine | 41% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
| 6-Methyluracil | 18% inhibition at 5 mM with thymine as substrate | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
thymine | - |
Neurospora crassa | |
| 0.22 | - |
5-Formyluracil | - |
Neurospora crassa | |
| 0.25 | - |
6-Azathymine | - |
Neurospora crassa | |
| 0.45 | - |
1-methylthymine | - |
Neurospora crassa | |
| 0.5 | - |
1-Methyluracil | - |
Neurospora crassa | |
| 0.6 | - |
1-ethylthymine | - |
Neurospora crassa | |
| 1 | - |
5-Hydroxymethyluracil | - |
Neurospora crassa | |
| 1 | - |
1-ethyluracil | - |
Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formyluracil + 2-oxoglutarate + O2 | Neurospora crassa | - |
5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-formyluracil + 2-oxoglutarate + O2 | Neurospora crassa STA 4 | - |
5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | Neurospora crassa | - |
5-formyluracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | Neurospora crassa STA 4 | - |
5-formyluracil + succinate + CO2 | - |
? | |
| thymine + 2-oxoglutarate + O2 | Neurospora crassa | - |
5-hydroxymethyluracil + succinate + CO2 | - |
? | |
| thymine + 2-oxoglutarate + O2 | Neurospora crassa STA 4 | - |
5-hydroxymethyluracil + succinate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
STA 4 | - |
| Neurospora crassa STA 4 | - |
STA 4 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-ethylthymine + 2-oxoglutarate + O2 | - |
Neurospora crassa | ? + succinate + CO2 | - |
? | |
| 1-ethyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa | ? + succinate + CO2 | - |
? | |
| 1-methylthymine + 2-oxoglutarate + O2 | - |
Neurospora crassa | 1-methyl-5-(hydroxymethyl)uracil + thymine + formaldehyde + succinate + CO2 | - |
? | |
| 1-methylthymine + 2-oxoglutarate + O2 | - |
Neurospora crassa STA 4 | 1-methyl-5-(hydroxymethyl)uracil + thymine + formaldehyde + succinate + CO2 | - |
? | |
| 1-methyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa | ? + succinate + CO2 | - |
? | |
| 5-aminouracil + 2-oxoglutarate + O2 | - |
Neurospora crassa | ? + succinate + CO2 | - |
? | |
| 5-aminouracil + 2-oxoglutarate + O2 | - |
Neurospora crassa STA 4 | ? + succinate + CO2 | - |
? | |
| 5-bromo-1-methyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa | ? + succinate + CO2 | - |
? | |
| 5-formyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa | 5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-formyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa STA 4 | 5-carboxyuracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa | 5-formyluracil + succinate + CO2 | - |
? | |
| 5-hydroxymethyluracil + 2-oxoglutarate + O2 | - |
Neurospora crassa STA 4 | 5-formyluracil + succinate + CO2 | - |
? | |
| 6-azathymine + 2-oxoglutarate + O2 | - |
Neurospora crassa | ? + succinate + CO2 | - |
? | |
| thymine + 2-oxoglutarate + O2 | - |
Neurospora crassa | 5-hydroxymethyluracil + succinate + CO2 | - |
? | |
| thymine + 2-oxoglutarate + O2 | - |
Neurospora crassa STA 4 | 5-hydroxymethyluracil + succinate + CO2 | - |
? | |
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