Crystallization (Comment) | Organism |
---|---|
mutant E146A/K147A, predicted to enhance crystallizability, to 2.7 A resolution, space group P43212 with a single protein molecule in the asymmetric unit. The N-terminal domain contains the signature DSBH or beta-barrel that is characteristic of 2OG-dependent oxygenases. The core DSBH is made up of two beta-sheets, one composed of five strands and the second one composed of three strands | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E146A/K147A | mutation predicted to enhance crystallizability | Escherichia coli |
R140A | loss of binding of 2-oxoglutarate | Escherichia coli |
S116A | loss of binding of 2-oxoglutarate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P27431 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | binding of 2-oxoglutarate to isoform YcfD occurs in an exothermic manner, dissociation constant is 55.6 microM. Residues S116 and R140 are involved in binding | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
50S ribosomal protein L16 arginine hydroxylase | - |
Escherichia coli |
ycfD | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | overexpression of L16 arginine hydroxylase YcfD inhibits cell growth signifying a toxic effect on ribosome assembly. The enzyme interacts with ribosomal protein RL-16 of the 50S subunit | Escherichia coli |