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Literature summary for 1.14.11.4 extracted from

  • Passoja, K.; Rautavuoma, K.; Ala-Kokko, L.; Kosonen, T.; Kivirikko, K.I.
    Cloning and characterization of a third human lysyl hydroxylase isoform (1998), Proc. Natl. Acad. Sci. USA, 95, 10482-10486.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.11.4

Application

Application Comment Organism
medicine deficiency in enzyme activity causes the Ehler-Danlos syndrome type 6 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cells using a baculovirus vector Homo sapiens
expression in various human tissues Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-oxoglutarate lysyl hydroxylases 1 and 3 Homo sapiens
0.3
-
 ascorbate lysyl hydroxylase 3 Homo sapiens
0.35
-
 ascorbate lysyl hydroxylase 1 Homo sapiens
0.5
-
 Ala-Arg-Gly-Ile-Lys-Gly-Ile-Arg-Gly-Phe-Ser-Gly lysyl hydroxylase 1 Homo sapiens
0.6
-
 Ala-Arg-Gly-Ile-Lys-Gly-Ile-Arg-Gly-Phe-Ser-Gly lysyl hydroxylase 3 Homo sapiens
0.7
-
 (Ile-Lys-Gly)3 lysyl hydroxylase 1 Homo sapiens
0.8
-
 (Ile-Lys-Gly)3 lysyl hydroxylase 3 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82380
-
 2 * 82380, calculation from amino acid sequence, recombinant enzyme: isoform 3 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen + 2-oxoglutarate + O2 Homo sapiens enzyme required during collagen biosynthesis 5-hydroxylysyl-collagen + succinate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60568
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification hydroxylation of lysyl residue in -X-Lys-Gly Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information existence of isoforms Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ile-Lys-Gly)3 + 2-oxoglutarate + O2
-
 Homo sapiens (Ile-5-hydroxylyseine-Gly)3 + succinate + CO2
-
 ?
2-oxoglutarate + O2 + ascorbate
-
 Homo sapiens succinate + CO2 + dehydroascorbate + H2O
-
 ?
Ala-Arg-Gly-Ile-Lys-Gly-Ile-Arg-Gly-Phe-Ser-Gly + 2-oxoglutarate + O2
-
 Homo sapiens Ala-Arg-Gly-Ile-5-hydroxylysine-Gly-Ile-Arg-Gly-Phe-Ser-Gly + succinate + CO2
-
 ?
collagen + 2-oxoglutarate + O2 enzyme required during collagen biosynthesis Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
 ?
peptidyl-L-lysine + 2-oxoglutarate + O2 synthetic peptides Homo sapiens peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?

Subunits

Subunits Comment Organism
homodimer 2 * 82380, calculation from amino acid sequence, recombinant enzyme: isoform 3 Homo sapiens

Synonyms

Synonyms Comment Organism
lysyl hydroxylase lysyl hydroxylase 3 Homo sapiens