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Literature summary for 1.14.11.4 extracted from

  • Valtavaara, M.; Papponen, H.; Pirttilae, A.M.; Hiltunen, K.; Helander, H.; Myllylae, R.
    Cloning and characterization of a novel human lysyl hydroxylase isoform highly expressed in pancreas and muscle (1997), J. Biol. Chem., 272, 6831-6834.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cells using a baculovirus vector Homo sapiens
expression in various human tissues Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required, Km: 0.001-0.005 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
2 * 88000 or 97000 SDS-PAGE, recombinant enzymes: isoform 2, possibly two forms are due to variation in the glycosylation of enzymes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen + 2-oxoglutarate + O2 Homo sapiens enzyme required during collagen biosynthesis 5-hydroxylysyl-collagen + succinate + CO2
-
?
collagen + 2-oxoglutarate + O2 Homo sapiens influence the integrity and stability of collagen 5-hydroxylysyl-collagen + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00469
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens
side-chain modification hydroxylation of lysyl residue in -X-Lys-Gly Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information existence of isoforms Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
collagen + 2-oxoglutarate + O2 enzyme required during collagen biosynthesis Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
?
collagen + 2-oxoglutarate + O2 influence the integrity and stability of collagen Homo sapiens 5-hydroxylysyl-collagen + succinate + CO2
-
?
peptidyl-L-lysine + 2-oxoglutarate + O2 synthetic peptides Homo sapiens peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?

Subunits

Subunits Comment Organism
homodimer 2 * 88000 or 97000 SDS-PAGE, recombinant enzymes: isoform 2, possibly two forms are due to variation in the glycosylation of enzymes Homo sapiens

Synonyms

Synonyms Comment Organism
lysyl hydroxylase lysyl hydroxylase 1 and 2 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ascorbate required Homo sapiens