Cloned (Comment) | Organism |
---|---|
isozymes CS1 and CS2, overexpression in Escherichia coli JM101 | Streptomyces clavuligerus |
General Stability | Organism |
---|---|
Co2+ completely inhibits the enzyme, competitive to Fe2+, but also stabilizes the enzyme against self-inactivation in absence of proclavaminate | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | complete inhibition competitive to Fe2+, but stabilizes the enzyme against self-inactivation in absence of proclavaminate | Streptomyces clavuligerus | |
diethyl dicarbonate | isozyme CS2, rapid inactivation at pH 6.0, 25°C, t1/2: 1.1 min | Streptomyces clavuligerus | |
N-ethylmaleimide | inactivation | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
proclavaminate | isozyme CS2, pH 7.0, 22°C | Streptomyces clavuligerus | |
0.22 | - |
proclavaminate | isozyme CS1, pH 7.0, 22°C | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Streptomyces clavuligerus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35360 | - |
recombinant enzyme | Streptomyces clavuligerus |
35360 | - |
CS1, mass spectrometry | Streptomyces clavuligerus |
35750 | - |
recombinant enzyme | Streptomyces clavuligerus |
35750 | - |
CS2, mass spectrometry | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
guanidinoproclavaminate + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
2 isozymes CS1 and CS2 | - |
Oxidation Stability | Organism |
---|---|
inactivation due to release of peroxide from reaction of 2-oxoglutarate and O2 in absence of proclavaminate, in presence of Fe2+, t1/2: 5 min, with ascorbate instead of 2-oxoglutarate t1/2: 50 min, catalase protects | Streptomyces clavuligerus |
Purification (Comment) | Organism |
---|---|
recombinant isozymes CS1 and CS2 to homogeneity, 10fold | Streptomyces clavuligerus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus | |
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | reaction mechanism | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.8 | - |
purified recombinant isozyme CS2 | Streptomyces clavuligerus |
0.86 | - |
purified recombinant isozyme CS1 | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
deoxyguanidinoproclavaminate + 2-oxoglutarate + O2 | hydroxylation | Streptomyces clavuligerus | guanidinoproclavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
additional information | 1 or more histidine as well as cysteine residue located at or near the active site or in the metal binding site, critical for catalysis | Streptomyces clavuligerus | ? | - |
? | |
additional information | dependent on O2 and 2-oxoglutarate | Streptomyces clavuligerus | ? | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CS | - |
Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces clavuligerus |