Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | inactivation due to release of peroxide from reaction of ascorbate and O2 in absence of proclavaminate, but in presence of Fe2+, t1/2: 50 min, catalase protects | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Streptomyces clavuligerus | |
0.0019 | - |
Fe2+ | pH 7.0, 22°C | Streptomyces clavuligerus | |
0.042 | - |
2-oxoglutarate | pH 7.0, 22°C | Streptomyces clavuligerus | |
0.19 | - |
(2S,3R)-proclavaminate | pH 7.0, 22°C | Streptomyces clavuligerus | |
0.38 | - |
rac-proclavaminate | pH 7.0, 22°C | Streptomyces clavuligerus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Streptomyces clavuligerus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
CS1, gel filtration | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
clavaminate + succinate + CO2 + H2O | - |
? | |
additional information | Streptomyces clavuligerus | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | ? | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | Streptomyces clavuligerus | - |
dihydroclavaminate + succinate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
strain ATCC 27064 | - |
Oxidation Stability | Organism |
---|---|
inactivation due to release of peroxide from reaction of 2-oxoglutarate and O2 in absence of proclavaminate, in presence of Fe2+, t1/2: 5 min, with ascorbate instead of 2-oxoglutarate t1/2: 50 min, catalase protects | Streptomyces clavuligerus |
Purification (Comment) | Organism |
---|---|
92fold | Streptomyces clavuligerus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
dihydroclavaminate + 2-oxoglutarate + O2 = clavaminate + succinate + CO2 + H2O | kinetic mechanism | Streptomyces clavuligerus | |
proclavaminate + 2-oxoglutarate + O2 = dihydroclavaminate + succinate + CO2 + H2O | kinetic mechanism | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.57 | - |
purified enzyme, 40°C | Streptomyces clavuligerus |
Storage Stability | Organism |
---|---|
-70°C, purified enzyme, frozen in liquid N2, stable for at least several months | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3R)-proclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
r | |
2-oxoglutarate + O2 | side reaction in absence of proclavaminate, oxidative decarboxylation of 2-oxoglutarate in presence of Fe2+ and formation of peroxide, which inactivates the enzyme | Streptomyces clavuligerus | ? + H2O2 | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
dihydroclavaminate + 2-oxoglutarate + O2 | cyclization | Streptomyces clavuligerus | clavaminate + succinate + CO2 + H2O | - |
? | |
additional information | specific for 2-oxoglutarate | Streptomyces clavuligerus | ? | - |
? | |
additional information | stereospecificity | Streptomyces clavuligerus | ? | - |
? | |
additional information | biosynthesic pathway of clavulanic acid, a beta-lactamase inhibitor | Streptomyces clavuligerus | ? | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | saturation | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | no activity with the racemic erythro isomers (2S,3S) and (2R,3R) | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? | |
proclavaminate + 2-oxoglutarate + O2 | specific for the threo-(2S,3R) enantiomer | Streptomyces clavuligerus | dihydroclavaminate + succinate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1* 47000, enzyme form 1, SDS-PAGE | Streptomyces clavuligerus |
monomer | 1* 46000, enzyme form 2, SDS-PAGE | Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.725 | - |
rac-proclavaminate | pH 7.0, 40°C | Streptomyces clavuligerus | |
0.765 | - |
(2S,3R)-proclavaminate | pH 7.0, 40°C | Streptomyces clavuligerus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces clavuligerus |