Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5,7-dimethyl-5,6,7,8-tetrahydropterin | partial activation | Hyoscyamus niger | |
ascorbate | 4 mM, 5.9fold stimulation | Hyoscyamus niger | |
catalase | activation | Hyoscyamus niger | |
dehydroascorbate | partial activation | Hyoscyamus niger | |
dithiothreitol | partial activation | Hyoscyamus niger | |
isoascorbate | partial activation | Hyoscyamus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.1 mM, 35% inhibition | Hyoscyamus niger | |
2,2'-dipyridyl | 0.1 mM, 15% inhibition | Hyoscyamus niger | |
3,4-dihydroxybenzoate | - |
Hyoscyamus niger | |
3,4-dihydroxycinnamate | - |
Hyoscyamus niger | |
3,4-dihydroxymandelate | - |
Hyoscyamus niger | |
3,4-Dihydroxyphenylacetate | - |
Hyoscyamus niger | |
3,4-Dihydroxyphenylpropionate | - |
Hyoscyamus niger | |
3-oxoglutaric acid | - |
Hyoscyamus niger | |
5,7-dehydrohyoscyamine | - |
Hyoscyamus niger | |
Apoatropine | - |
Hyoscyamus niger | |
Bathocuproine | 0.1 mM, 27% inhibition | Hyoscyamus niger | |
Ca2+ | 0.4 mM, 11% inhibition | Hyoscyamus niger | |
Cd2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
Co2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
Cu2+ | 0.4 mM, complete inhibition | Hyoscyamus niger | |
diethyldithiocarbamate | 0.1 mM, 37% inhibition | Hyoscyamus niger | |
EDTA | 0.1 mM, 67% inhibition | Hyoscyamus niger | |
Fe3+ | 0.4 mM, 28% inhibition | Hyoscyamus niger | |
fumarate | - |
Hyoscyamus niger | |
Hg2+ | 0.4 mM, 96% inhibition | Hyoscyamus niger | |
L-Homatropine | - |
Hyoscyamus niger | |
Mn2+ | 0.4 mM, 95% inhibition | Hyoscyamus niger | |
Ni2+ | 0.4 mM, 96% inhibition | Hyoscyamus niger | |
nitroblue tetrazolium | - |
Hyoscyamus niger | |
Noratropine-N-acetic acid | - |
Hyoscyamus niger | |
Phenylacetyltropine | - |
Hyoscyamus niger | |
pyridine 2,3-dicarboxylate | competitive with respect to 2-oxoglutarate | Hyoscyamus niger | |
Pyridine 2,4-dicarboxylate | competitive with respect to 2-oxoglutarate | Hyoscyamus niger | |
Pyridine 3,4-dicarboxylate | - |
Hyoscyamus niger | |
Tiron | 0.1 mM, 44% inhibition | Hyoscyamus niger | |
Zn2+ | 0.4 mM, complete inhibition | Hyoscyamus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
6,7-Dehydrohyoscyamine | - |
Hyoscyamus niger | |
0.035 | - |
L-hyoscyamine | - |
Hyoscyamus niger | |
0.043 | - |
2-oxoglutarate | - |
Hyoscyamus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates | Hyoscyamus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 39000 + x * 19000, SDS-PAGE | Hyoscyamus niger |
39000 | - |
x * 39000 + x * 19000, SDS-PAGE | Hyoscyamus niger |
41000 | - |
gel filtration | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-hyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | enzyme is involved in biosynthetic pathway leading to scopolamine | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | culture | Hyoscyamus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Hyoscyamus niger |
Storage Stability | Organism |
---|---|
-20°C, stable for more than 3 months | Hyoscyamus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 | 15% of the activity with L-hyoscyamine | Hyoscyamus niger | 2-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 | - |
? | |
2-oxoglutarate + p-hydroxyatropine + O2 | 26% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
2-oxoglutarate + trans-cinnamoyltropine + O2 | 39% of the activity with L-hyoscyamine | Hyoscyamus niger | succinate + trans-cinnamoyl-6-hydroxytropine + CO2 | - |
? | |
3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 | 56% of the activity with L-hyoscyamine | Hyoscyamus niger | 3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2 | - |
? | |
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 | 119% of the activity with L-hyoscyamine | Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
apoatropine + 2-oxoglutarate + O2 | 45% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxyapoatropine + succinate + CO2 | - |
? | |
isobutyltropine + 2-oxoglutarate + O2 | 15% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
L-homatropine + 2-oxoglutarate + O2 | 81% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxyhomatropine + succinate + CO2 | - |
? | |
L-hyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
L-hyoscyamine + 2-oxoglutarate + O2 | enzyme is involved in biosynthetic pathway leading to scopolamine | Hyoscyamus niger | 6beta-hydroxyhyoscyamine + succinate + CO2 | - |
? | |
L-norhyoscyamine + 2-oxoglutarate + O2 | 81% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxynorhyoscyamine + succinate + CO2 | - |
? | |
additional information | high specificity for 2-oxoglutarate in hydroxylation | Hyoscyamus niger | ? | - |
? | |
noratropine-N-acetic acid + 2-oxoglutarate + O2 | 17% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? | |
phenylacetyltropine + 2-oxoglutarate + O2 | 81% of the activity with L-hyoscyamine | Hyoscyamus niger | 6-hydroxyphenylacetyltropine + succinate + CO2 | - |
? | |
phenylalanyltropine + 2-oxoglutarate + O2 | 8% of the activity with L-hyoscyamine | Hyoscyamus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000 + x * 19000, SDS-PAGE | Hyoscyamus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Hyoscyamus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 50% of maximal activity at pH 6.0 and pH 9.0 | Hyoscyamus niger |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
Pyridine 2,4-dicarboxylate | - |
Hyoscyamus niger | |
0.09 | - |
Pyridine 3,4-dicarboxylate | - |
Hyoscyamus niger |