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Literature summary for 1.14.11.11 extracted from

  • Hashimoto, T.; Yamada, Y.
    Purification and characterization of hyoscyamine 6 beta-hydroxylase from root cultures of Hyoscyamus niger L. Hydroxylase and epoxidase activities in the enzyme preparation (1987), Eur. J. Biochem., 164, 277-285.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5,7-dimethyl-5,6,7,8-tetrahydropterin partial activation Hyoscyamus niger
ascorbate 4 mM, 5.9fold stimulation Hyoscyamus niger
catalase activation Hyoscyamus niger
dehydroascorbate partial activation Hyoscyamus niger
dithiothreitol partial activation Hyoscyamus niger
isoascorbate partial activation Hyoscyamus niger

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.1 mM, 35% inhibition Hyoscyamus niger
2,2'-dipyridyl 0.1 mM, 15% inhibition Hyoscyamus niger
3,4-dihydroxybenzoate
-
Hyoscyamus niger
3,4-dihydroxycinnamate
-
Hyoscyamus niger
3,4-dihydroxymandelate
-
Hyoscyamus niger
3,4-Dihydroxyphenylacetate
-
Hyoscyamus niger
3,4-Dihydroxyphenylpropionate
-
Hyoscyamus niger
3-oxoglutaric acid
-
Hyoscyamus niger
5,7-dehydrohyoscyamine
-
Hyoscyamus niger
Apoatropine
-
Hyoscyamus niger
Bathocuproine 0.1 mM, 27% inhibition Hyoscyamus niger
Ca2+ 0.4 mM, 11% inhibition Hyoscyamus niger
Cd2+ 0.4 mM, complete inhibition Hyoscyamus niger
Co2+ 0.4 mM, complete inhibition Hyoscyamus niger
Cu2+ 0.4 mM, complete inhibition Hyoscyamus niger
diethyldithiocarbamate 0.1 mM, 37% inhibition Hyoscyamus niger
EDTA 0.1 mM, 67% inhibition Hyoscyamus niger
Fe3+ 0.4 mM, 28% inhibition Hyoscyamus niger
fumarate
-
Hyoscyamus niger
Hg2+ 0.4 mM, 96% inhibition Hyoscyamus niger
L-Homatropine
-
Hyoscyamus niger
Mn2+ 0.4 mM, 95% inhibition Hyoscyamus niger
Ni2+ 0.4 mM, 96% inhibition Hyoscyamus niger
nitroblue tetrazolium
-
Hyoscyamus niger
Noratropine-N-acetic acid
-
Hyoscyamus niger
Phenylacetyltropine
-
Hyoscyamus niger
pyridine 2,3-dicarboxylate competitive with respect to 2-oxoglutarate Hyoscyamus niger
Pyridine 2,4-dicarboxylate competitive with respect to 2-oxoglutarate Hyoscyamus niger
Pyridine 3,4-dicarboxylate
-
Hyoscyamus niger
Tiron 0.1 mM, 44% inhibition Hyoscyamus niger
Zn2+ 0.4 mM, complete inhibition Hyoscyamus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
6,7-Dehydrohyoscyamine
-
Hyoscyamus niger
0.035
-
L-hyoscyamine
-
Hyoscyamus niger
0.043
-
2-oxoglutarate
-
Hyoscyamus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Hyoscyamus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 39000 + x * 19000, SDS-PAGE Hyoscyamus niger
39000
-
x * 39000 + x * 19000, SDS-PAGE Hyoscyamus niger
41000
-
gel filtration Hyoscyamus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-hyoscyamine + 2-oxoglutarate + O2 Hyoscyamus niger enzyme is involved in biosynthetic pathway leading to scopolamine 6beta-hydroxyhyoscyamine + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Hyoscyamus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
root culture Hyoscyamus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Hyoscyamus niger

Storage Stability

Storage Stability Organism
-20°C, stable for more than 3 months Hyoscyamus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 15% of the activity with L-hyoscyamine Hyoscyamus niger 2-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2
-
?
2-oxoglutarate + p-hydroxyatropine + O2 26% of the activity with L-hyoscyamine Hyoscyamus niger ?
-
?
2-oxoglutarate + trans-cinnamoyltropine + O2 39% of the activity with L-hyoscyamine Hyoscyamus niger succinate + trans-cinnamoyl-6-hydroxytropine + CO2
-
?
3-hydroxy-3-phenylpropionyltropine + 2-oxoglutarate + O2 56% of the activity with L-hyoscyamine Hyoscyamus niger 3-hydroxy-3-phenylpropionyl-6-hydroxytropine + succinate + CO2
-
?
6,7-dehydrohyoscyamine + 2-oxoglutarate + O2 119% of the activity with L-hyoscyamine Hyoscyamus niger scopolamine + succinate + CO2
-
?
apoatropine + 2-oxoglutarate + O2 45% of the activity with L-hyoscyamine Hyoscyamus niger 6-hydroxyapoatropine + succinate + CO2
-
?
isobutyltropine + 2-oxoglutarate + O2 15% of the activity with L-hyoscyamine Hyoscyamus niger ?
-
?
L-homatropine + 2-oxoglutarate + O2 81% of the activity with L-hyoscyamine Hyoscyamus niger 6-hydroxyhomatropine + succinate + CO2
-
?
L-hyoscyamine + 2-oxoglutarate + O2
-
Hyoscyamus niger 6beta-hydroxyhyoscyamine + succinate + CO2
-
?
L-hyoscyamine + 2-oxoglutarate + O2 enzyme is involved in biosynthetic pathway leading to scopolamine Hyoscyamus niger 6beta-hydroxyhyoscyamine + succinate + CO2
-
?
L-norhyoscyamine + 2-oxoglutarate + O2 81% of the activity with L-hyoscyamine Hyoscyamus niger 6-hydroxynorhyoscyamine + succinate + CO2
-
?
additional information high specificity for 2-oxoglutarate in hydroxylation Hyoscyamus niger ?
-
?
noratropine-N-acetic acid + 2-oxoglutarate + O2 17% of the activity with L-hyoscyamine Hyoscyamus niger ?
-
?
phenylacetyltropine + 2-oxoglutarate + O2 81% of the activity with L-hyoscyamine Hyoscyamus niger 6-hydroxyphenylacetyltropine + succinate + CO2
-
?
phenylalanyltropine + 2-oxoglutarate + O2 8% of the activity with L-hyoscyamine Hyoscyamus niger ?
-
?

Subunits

Subunits Comment Organism
? x * 39000 + x * 19000, SDS-PAGE Hyoscyamus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Hyoscyamus niger

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 50% of maximal activity at pH 6.0 and pH 9.0 Hyoscyamus niger

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.009
-
Pyridine 2,4-dicarboxylate
-
Hyoscyamus niger
0.09
-
Pyridine 3,4-dicarboxylate
-
Hyoscyamus niger