Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is activated 1.5fold and 3fold in presence of 8 M urea and 0.5% SDS respectively | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | weak | Pseudomonas sp. | |
hydroxylamine | - |
Pseudomonas sp. | |
KCN | - |
Pseudomonas sp. | |
Sodium azide | weak | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
L-tryptophan | at pH 6.0 | Pseudomonas sp. | |
0.004 | - |
L-tryptophan | at pH 3.0 | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.3 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-80°C, several months | Pseudomonas sp. |
0°C, pH 6, stable in presence of either 80% ethyl alcohol, 6 M urea or 0.5% SDS for at least 6 h | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-indoleacetamide + O2 | - |
Pseudomonas sp. | ? | - |
? | |
3-indolelactate + O2 | - |
Pseudomonas sp. | ? | - |
? | |
3-indolepropionate + O2 | - |
Pseudomonas sp. | ? | - |
? | |
3-indolethanol + O2 | - |
Pseudomonas sp. | ? | - |
? | |
3-methylindole + O2 | - |
Pseudomonas sp. | 3-indolecarboxaldehyde + H2O | - |
? | |
D-tryptophan + O2 | - |
Pseudomonas sp. | ? | - |
? | |
L-tryptophan + O2 | - |
Pseudomonas sp. | 3-indoleglycolaldehyde + CO2 + NH3 | - |
? | |
melatonin + O2 | - |
Pseudomonas sp. | ? | - |
? | |
additional information | overview: specificity | Pseudomonas sp. | additional information | - |
? | |
N-acetyl-L-tryptophan + O2 | - |
Pseudomonas sp. | ? | - |
? | |
N-acetyl-L-tryptophanamide + O2 | - |
Pseudomonas sp. | N-acetyl-alpha,beta-didehydrotryptophanamide + H2O | - |
? | |
N-acetylacetamide + O2 | - |
Pseudomonas sp. | ? | - |
? | |
tryptamine + O2 | - |
Pseudomonas sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Pseudomonas sp. |
6 | - |
- |
Pseudomonas sp. |
11 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | hemoprotein | Pseudomonas sp. |