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Literature summary for 1.13.12.4 extracted from

  • Durfor, C.N.; Cromartie, T.H.
    Inactivation of L-lactate monooxygenase by nitration with tetranitromethane (1981), Arch. Biochem. Biophys., 210, 710-716.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
(R)-lactate
-
Mycolicibacterium phlei
acetate
-
Mycolicibacterium phlei
oxalate
-
Mycolicibacterium phlei
p-chloromercuribenzoate 100% inhibition at 1.1 mM at pH 8 and 25°C after 30 min, excess of 2-mercaptoethanol protects Mycolicibacterium phlei
Tetranitromethane 20 min at pH 8.0 and 1.5 mM; rapid and irreversible inactivation at 30°C, nitration of a single tyrosine per subunit, competitive inhibitors such as acetate, (R)-lactate or oxalate protect from inactivation Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + O2 Mycolicibacterium phlei
-
acetate + CO2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium phlei
-
-
-

Storage Stability

Storage Stability Organism
4°C, 80% saturated ammonium sulfate in the dark Mycolicibacterium phlei
4°C, crystalline suspension in 1 M sodium acetate buffer, pH 5.4, in the dark Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + O2
-
Mycolicibacterium phlei acetate + CO2 + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
FMN
-
Mycolicibacterium phlei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
oxalate
-
Mycolicibacterium phlei
4
-
(R)-lactate
-
Mycolicibacterium phlei
11
-
acetate
-
Mycolicibacterium phlei