Cloned (Comment) | Organism |
---|---|
the coding region for the enzyme component StaP is cloned into expression vector pET26b(+) (Novagen, San Diego, CA), with a C-terminal 6 * His tag, and overexpressed in Escherichia coli strain BL21 | Streptomyces sp. |
Crystallization (Comment) | Organism |
---|---|
X-ray crystal structures of chromopyrrolic acid-bound and chromopyrrolic acid-free forms of enzyme component StaP. The enzyme attains a more ordered conformation upon binding of the substrate. Substantial conformational changes are observed at and around the substrate-binding pocket to optimize interactions with the enzyme. The enzymechromopyrrolic acid complex structure reveals that chromopyrrolic acid has a twisted-butterfly shape in the active site, which is perpendicular to the heme plane | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | Streptomyces sp. | staurosporine biosynthesis | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | Streptomyces sp. TP-A0274 | staurosporine biosynthesis | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | Q83WG3 | TP-A0274 | - |
Streptomyces sp. TP-A0274 | Q83WG3 | TP-A0274 | - |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | - |
Streptomyces sp. | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | staurosporine biosynthesis | Streptomyces sp. | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | - |
Streptomyces sp. TP-A0274 | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? | |
dichlorochromopyrrolate + 4 O2 + 4 NADH + 4 H+ | staurosporine biosynthesis | Streptomyces sp. TP-A0274 | dichloroarcyriaflavin A + 2 CO2 + 6 H2O + 4 NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP245A1 | - |
Streptomyces sp. |
cytochrome P450 StaP | - |
Streptomyces sp. |
StaP | - |
Streptomyces sp. |