Application | Comment | Organism |
---|---|---|
degradation | enzyme exerts a synergistic effect on the efficiency of polyisoprene cleavage by rubber oxidase RoxA, EC 1.13.11.85 | Xanthomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diethyl dithiocarbamate | - |
Xanthomonas sp. | |
imidazole | - |
Xanthomonas sp. | |
additional information | not inhibitory: EDTA up to 10 mM, carbon monoxide | Xanthomonas sp. | |
Pyridine | - |
Xanthomonas sp. | |
SDS | partial inhibition | Xanthomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas sp. | A0A1S6Q8F9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | calculated molecular mass is 73800 Da for precursor protein, and 70300 Da for mature protein | Xanthomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
pH 7.0, 23°C | Xanthomonas sp. |
5.7 | - |
pH 7.0, 30°C | Xanthomonas sp. |
6.4 | - |
pH 7.0, 37°C | Xanthomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-1,4-polyisoprene + n O2 | - |
Xanthomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73800, calculated for precursor protein, x * 70300, calculated for mature protein, x * 70300, SDS-PAGE | Xanthomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
RoxB | - |
Xanthomonas sp. |
rubber oxygenase B | - |
Xanthomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Xanthomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | presence of 2 c-type hemes, presence of two c-type heme binding motifs (C192HAC195H and C391ASC394H) | Xanthomonas sp. |
Organism | Comment | Expression |
---|---|---|
Xanthomonas sp. | expression is upregulated during growth on polyisoprene | up |
General Information | Comment | Organism |
---|---|---|
physiological function | synergistic effect of the simultaneous presence of RoxAXsp and RoxBXsp on the efficiency of polyisoprene cleavage | Xanthomonas sp. |