Cloned (Comment) | Organism |
---|---|
expression in the Escherichia coli KRX strain | Blepharisma stoltei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.589 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, wild-type enzyme | Blepharisma stoltei | |
0.622 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme N127T | Blepharisma stoltei | |
2.85 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme H127E | Blepharisma stoltei | |
13.6 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, wild-type enzyme | Blepharisma stoltei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | Blepharisma stoltei | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blepharisma stoltei | - |
- |
- |
Blepharisma stoltei | A0A286T905 | - |
- |
Blepharisma stoltei | A0A286TA52 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Blepharisma stoltei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | - |
Blepharisma stoltei | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2 | Glu132 in Blepharisma IDO-III is crucial for its high affinity for L-Trp. No activity with 5-hydroxy-L-tryptophan | Blepharisma stoltei | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2 | no activity with 5-hydroxy-L-tryptophan | Blepharisma stoltei | N-formyl-L-kynurenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO-I | - |
Blepharisma stoltei |
IDO-II | - |
Blepharisma stoltei |
IDO-III | - |
Blepharisma stoltei |
IDO-IV | - |
Blepharisma stoltei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme H127E | Blepharisma stoltei | |
1.47 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, wild-type enzyme | Blepharisma stoltei | |
3.9 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme N127T | Blepharisma stoltei | |
6.03 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme E132H | Blepharisma stoltei | |
6.5 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, wild-type enzyme | Blepharisma stoltei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme-containing enzyme | Blepharisma stoltei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, wild-type enzyme | Blepharisma stoltei | |
0.98 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme E132H | Blepharisma stoltei | |
6.3 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme N127T | Blepharisma stoltei | |
11 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, wild-type enzyme | Blepharisma stoltei | |
215 | - |
L-tryptophan | pH 8.0, temperature not specified in the publication, mutant enzyme H127E | Blepharisma stoltei |