Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | - |
Homo sapiens | |
cytochrome b5 | - |
Homo sapiens | |
methylene blue | - |
Homo sapiens | |
NADPH-cytochrome P450 reductase | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Methyltryptophan | - |
Homo sapiens | |
annulin B | - |
Homo sapiens | |
menadione | menadione effectively inhibits L-Trp oxidation from reactions supported with ascorbaic acid/methylene blue | Homo sapiens | |
methylene blue | addition of methylene blue (0-0.1 mM) to NADPH-cytochrome P450 reductase-supported D-Trp incubations (with or without cytochrome b5) leads to concentration-dependent inhibition of IDO activity, addition of methylene blue (0-0.03 mM) to NADPH-cytochrome P450 reductase-supported L-Trp oxidation reactions results in a switch from substrate inhibition kinetics to Michaelis-Menten with increasing methylene blue concentration decreasing the affinity of IDO for L-Trp | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00072 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0015 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and in the presence of 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0017 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0019 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0039 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.004 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0049 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0054 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0124 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0129 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0224 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
0.0279 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | Homo sapiens | - |
N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | Homo sapiens | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14902 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-tryptophan + O2 | - |
Homo sapiens | N-formyl-D-kynurenine | - |
? | |
L-tryptophan + O2 | - |
Homo sapiens | N-formyl-L-kynurenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDO | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
1.3 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
1.7 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and in the presence of 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
2.2 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
2.5 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
3 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
3.4 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
4.2 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
6.4 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
8.1 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
10 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
12 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00064 | - |
in PBS buffer (pH 7.4), at 37°C | Homo sapiens | menadione |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
290 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
490 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
540 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.03 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
610 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
640 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
740 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
760 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and in the absence of cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
800 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.01 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
850 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0005 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
1000 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the presence of 0.0001 mM methylene blue and 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
1100 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and in the presence of 250 nM cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens | |
1200 | - |
L-tryptophan | NADPH-cytochrome P450 reductase-supported oxidation, in the absence of methylene blue and cytochrome b5, in PBS buffer (pH 7.4), at 37°C | Homo sapiens |