Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-4-fluorotryptophan | - |
Delftia acidovorans | |
DL-4-fluorotryptophan | - |
Rattus norvegicus | |
DL-4-methyltryptophan | - |
Delftia acidovorans | |
DL-4-methyltryptophan | - |
Rattus norvegicus | |
DL-5-fluorotryptophan | - |
Delftia acidovorans | |
DL-5-fluorotryptophan | - |
Rattus norvegicus | |
DL-5-Methyltryptophan | - |
Delftia acidovorans | |
DL-5-Methyltryptophan | - |
Rattus norvegicus | |
indole | - |
Delftia acidovorans | |
indole | - |
Rattus norvegicus | |
Indolepropionic acid | - |
Delftia acidovorans | |
Indolepropionic acid | - |
Rattus norvegicus | |
L-5-hydroxytryptophan | - |
Delftia acidovorans | |
L-5-hydroxytryptophan | - |
Rattus norvegicus | |
additional information | no inhibition by D-5-hydroxytryptophan | Delftia acidovorans | |
additional information | no inhibition by D-5-hydroxytryptophan | Rattus norvegicus | |
tryptamine | - |
Delftia acidovorans | |
tryptamine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
O2 | pH 8.0 | Rattus norvegicus | |
0.038 | - |
O2 | pH 7.0 | Delftia acidovorans | |
0.25 | - |
L-Trp | pH 8.0 | Rattus norvegicus | |
0.3 | - |
L-Trp | pH 7.0 | Delftia acidovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | enzyme contains 2 copper moieties | Rattus norvegicus | |
copper | enzyme contains 2 copper moieties | Delftia acidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
4 * 31000, SDS-PAGE | Delftia acidovorans |
43000 | - |
alpha2beta2, 2 * 43000 + 2 * 43000, SDS-PAGE | Rattus norvegicus |
122000 | - |
gel filtration, sucrose density gradient centrifugation, equilibrium sedimentation | Delftia acidovorans |
167000 | - |
gel filtration, sucrose density gradient centrifugation, equilibrium sedimentation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Trp + O2 | Rattus norvegicus | regulation of enzyme activity | L-formylkynurenine | - |
? | |
L-Trp + O2 | Delftia acidovorans | regulation of enzyme activity | L-formylkynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Delftia acidovorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
- |
Rattus norvegicus |
17 | - |
- |
Delftia acidovorans |
Storage Stability | Organism |
---|---|
pH 7.0, anaerobically stored in 0.1 M potassium phosphate buffer containing L-Trp, quite stable | Rattus norvegicus |
pH 7.0, anaerobically stored in 0.1 M potassium phosphate buffer containing L-Trp, quite stable | Delftia acidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-6-fluorotryptophan + O2 | - |
Rattus norvegicus | ? | - |
? | |
DL-6-fluorotryptophan + O2 | - |
Delftia acidovorans | ? | - |
? | |
DL-6-methyltryptophan | - |
Rattus norvegicus | ? | - |
? | |
L-Trp + O2 | - |
Rattus norvegicus | L-formylkynurenine | - |
? | |
L-Trp + O2 | - |
Delftia acidovorans | L-formylkynurenine | - |
? | |
L-Trp + O2 | regulation of enzyme activity | Rattus norvegicus | L-formylkynurenine | - |
? | |
L-Trp + O2 | regulation of enzyme activity | Delftia acidovorans | L-formylkynurenine | - |
? | |
additional information | no activity with D-Trp | Rattus norvegicus | additional information | - |
? | |
additional information | no activity with D-Trp | Delftia acidovorans | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 31000, SDS-PAGE | Delftia acidovorans |
tetramer | alpha2beta2, 2 * 43000 + 2 * 43000, SDS-PAGE | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 2070 per min, formylkynurenine formed | Delftia acidovorans | |
additional information | - |
additional information | 420 per min, formylkynurenine formed | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | enzyme contains two heme moieties | Rattus norvegicus | |
heme | enzyme contains two heme moieties | Delftia acidovorans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
L-5-hydroxytryptophan | - |
Delftia acidovorans | |
0.01 | - |
L-5-hydroxytryptophan | - |
Rattus norvegicus | |
0.08 | - |
Indolepropionic acid | - |
Delftia acidovorans | |
0.1 | - |
tryptamine | - |
Delftia acidovorans | |
0.12 | - |
DL-5-fluorotryptophan | - |
Rattus norvegicus | |
0.14 | - |
indole | - |
Rattus norvegicus | |
0.19 | - |
DL-5-Methyltryptophan | - |
Rattus norvegicus | |
0.21 | - |
Indolepropionic acid | - |
Delftia acidovorans | |
0.28 | - |
DL-4-methyltryptophan | - |
Rattus norvegicus | |
0.33 | - |
DL-4-fluorotryptophan | - |
Rattus norvegicus | |
0.39 | - |
DL-5-fluorotryptophan | - |
Delftia acidovorans | |
0.55 | - |
DL-5-Methyltryptophan | - |
Delftia acidovorans | |
0.67 | - |
DL-4-methyltryptophan | - |
Delftia acidovorans | |
1 | - |
DL-4-fluorotryptophan | - |
Delftia acidovorans | |
1.8 | - |
tryptamine | - |
Delftia acidovorans | |
2.7 | - |
indole | - |
Delftia acidovorans |