Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required for maximal activity at pH 6 | Pseudomonas fluorescens | |
ascorbate | required for maximal activity at pH 7 | Bos taurus | |
glutathione | required for maximal activity at pH 6 and 5.4 | Pseudomonas fluorescens | |
glutathione | reduced glutathione is required for maximal activity at pH 7 | Bos taurus | |
additional information | pH 7.0: a combination of ascorbate, reduced glutathione and ferrous iron is required for activation, pH 5.3: ferrous iron alone fully activates | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
General Stability | Organism |
---|---|
10% acetone stabilizes | Bos taurus |
unlike the mammalian enzyme, the bacterial enzyme is fairly stable on aging or during storage | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Lewisite | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
Methylmercuric bromide | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
p-chloromercuribenzoate | inhibits by competing with Fe2+ for a common enzymic binding site | Bos taurus | |
p-chloromercuribenzoate | competitive inhibition with respect to Fe2+ but not to homogentisate | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
homogentisate | at pH 6 | Pseudomonas fluorescens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | ferrous mercaptans at the active center | Bos taurus | |
Fe2+ | Fe2+ is required as cofactor | Pseudomonas fluorescens | |
Fe2+ | ferrous ion is required for maximal activity at pH 7 and pH 5.3 | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
380000 | - |
ultracentrifugal analysis | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Oxidation Stability | Organism |
---|---|
Fe2+, organic mercurials and reducing agents protect the enzyme from irreversible aerobic oxidation | Bos taurus |
Purification (Comment) | Organism |
---|---|
partial purification | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
homogentisate + O2 | - |
Pseudomonas fluorescens | 4-maleylacetoacetate | - |
? |