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Literature summary for 1.13.11.46 extracted from

  • Choroba, O.W.; Williams, D.H.; Spencer, J.B.
    Biosynthesis of the vancomycin group of antibiotics: involvement of an unusual dioxygenase in the pathway to (S)-4-hydroxyphenylglycine (2000), J. Am. Chem. Soc., 122, 5389-5390.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Amycolatopsis orientalis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ putative FeIV=O species hydroxylates benzylic position Amycolatopsis orientalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40368
-
alpha,alpha, 2 * 40368, gel filtration, electrospray mass spectrometry Amycolatopsis orientalis
80000
-
gel filtration, electrospray mass spectrometry Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylpyruvate + O2 Amycolatopsis orientalis
-
4-hydroxymandelate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein, Ni-NTA resin Amycolatopsis orientalis

Reaction

Reaction Comment Organism Reaction ID
4-hydroxyphenylpyruvate + O2 = (S)-4-hydroxymandelate + CO2 one oxygen from molecular oxygen is incorporated into the carboxyl group and one into the benzylic hydroxyl group Amycolatopsis orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
Amycolatopsis orientalis 4-hydroxymandelate + CO2
-
?

Subunits

Subunits Comment Organism
dimer alpha,alpha, 2 * 40368, gel filtration, electrospray mass spectrometry Amycolatopsis orientalis