KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
3'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.001 | - |
2,3-Dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.001 | - |
2'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.0015 | - |
4'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.01 | - |
3'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.011 | - |
4'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.012 | - |
4'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.018 | - |
2'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.019 | - |
2'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.026 | - |
2,3-Dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.029 | - |
2,3-Dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.1 | - |
3-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
1.23 | - |
3-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
1.94 | - |
3-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
2.4 | - |
catechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
2.8 | - |
4-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
61 | - |
4-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
93 | - |
4-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Rhodococcus globerulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Rhodococcus globerulus |
29000 | - |
x * 29000, SDS-PAGE | Rhodococcus globerulus |
34000 | - |
x * 34000, SDS-PAGE | Rhodococcus globerulus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus globerulus | - |
- |
- |
Rhodococcus globerulus P6 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
50% loss of activity after 4.5 min in air-satured buffer | Rhodococcus globerulus |
50% loss of activity after 43 min in air-satured buffer | Rhodococcus globerulus |
50% loss of activity after 7.2 min in air-satured buffer | Rhodococcus globerulus |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus globerulus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rhodococcus globerulus |
20 | - |
- |
Rhodococcus globerulus |
30 | - |
- |
Rhodococcus globerulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2 | 49% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',3',5'-trichloro-2,3-dihydroxybiphenyl + O2 | 49% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus P6 | 2-hydroxy-6-oxo-6-(2',3',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 | 25% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 | 57% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 | 25% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus P6 | 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',3'-dichloro-2,3-dihydroxybiphenyl + O2 | 57% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus P6 | 2-hydroxy-6-oxo-6-(2',3'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',4'-dichloro-2,3-dihydroxybiphenyl + O2 | 33% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2',4'-dichloro-2,3-dihydroxybiphenyl + O2 | 63% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2'-chloro-2,3-dihydroxybiphenyl + O2 | - |
Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2'-chloro-2,3-dihydroxybiphenyl + O2 | 50% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(2'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
2,3-dihydroxybiphenyl + O2 | - |
Rhodococcus globerulus | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O | - |
? | |
3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2 | 11% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',4',5'-trichloro-2,3-dihydroxybiphenyl + O2 | 26% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',4',5'-trichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',4'-dichloro-2,3-dihydroxybiphenyl + O2 | 49% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',4'-dichloro-2,3-dihydroxybiphenyl + O2 | 59% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',4'-dichloro-2,3-dihydroxybiphenyl + O2 | 61% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',4'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',5'-dichloro-2,3-dihydroxybiphenyl + O2 | 15% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',5'-dichloro-2,3-dihydroxybiphenyl + O2 | 49% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3',5'-dichloro-2,3-dihydroxybiphenyl + O2 | 61% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3',5'-dichlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3'-chloro-2,3-dihydroxybiphenyl + O2 | - |
Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3'-chloro-2,3-dihydroxybiphenyl + O2 | 64% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3'-chloro-2,3-dihydroxybiphenyl + O2 | 71% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3'-chloro-2,3-dihydroxybiphenyl + O2 | 95% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(3'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
3-methylcatechol + O2 | - |
Rhodococcus globerulus | ? | - |
? | |
3-methylcatechol + O2 | - |
Rhodococcus globerulus P6 | ? | - |
? | |
4'-chloro-2,3-dihydroxybiphenyl + O2 | - |
Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
4'-chloro-2,3-dihydroxybiphenyl + O2 | 61% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
4'-chloro-2,3-dihydroxybiphenyl + O2 | 77% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
4'-chloro-2,3-dihydroxybiphenyl + O2 | 84% of activity with 2,3-dihydroxybiphenyl | Rhodococcus globerulus | 2-hydroxy-6-oxo-6-(4'-chlorophenyl)-hexa-2,4-dienoate + H2O | - |
? | |
4-methylcatechol + O2 | - |
Rhodococcus globerulus | ? | - |
? | |
catechol + O2 | - |
Rhodococcus globerulus | ? | - |
? | |
catechol + O2 | - |
Rhodococcus globerulus P6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Rhodococcus globerulus |
? | x * 34000, SDS-PAGE | Rhodococcus globerulus |
? | x * 29000, SDS-PAGE | Rhodococcus globerulus |
Synonyms | Comment | Organism |
---|---|---|
BphC1 | - |
Rhodococcus globerulus |
BphC2 | - |
Rhodococcus globerulus |
BphC3 | - |
Rhodococcus globerulus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
catechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.064 | - |
4-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.24 | - |
4-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.25 | - |
2'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.36 | - |
4-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
0.92 | - |
catechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
3.5 | - |
2'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
4 | - |
3'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
4.4 | - |
3-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
4.5 | - |
4'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
4.8 | - |
2,3-Dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
7.5 | - |
2'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
8.3 | - |
4'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
8.5 | - |
3'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
9.8 | - |
3-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
9.8 | - |
2,3-Dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
12 | - |
3'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
13.6 | - |
3-methylcatechol | 25°C, pH 7.5 | Rhodococcus globerulus | |
16.5 | - |
2,3-Dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus | |
18.8 | - |
4'-chloro-2,3-dihydroxybiphenyl | 25°C, pH 7.5 | Rhodococcus globerulus |