KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0471 | - |
O2 | cosubstrate N-terminal Cys of RGS5, pH not specified in the publication, 37°C | Homo sapiens | |
0.0551 | - |
O2 | cosubstrate N-terminal Cys of RGS4, pH not specified in the publication, 37°C | Homo sapiens | |
0.0715 | - |
N-terminal Cys of RGS5 | pH not specified in the publication, 37°C | Homo sapiens | |
0.123 | - |
N-terminal Cys of RGS4 | pH not specified in the publication, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96SZ5 | cf. EC 1.13.11.19 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic cancer cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-terminal Cys of RGS4 + O2 | i.e. regulator of G-protein signalling | Homo sapiens | N-terminal Cys-sulfinic acid of RGS4 | - |
? | |
N-terminal Cys of RGS5 + O2 | i.e. regulator of G-protein signalling | Homo sapiens | N-terminal Cys-sulfinic acid of RGS5 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADO | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.9 | - |
N-terminal Cys of RGS5 | pH not specified in the publication, 37°C | Homo sapiens | |
20.1 | - |
N-terminal Cys of RGS4 | pH not specified in the publication, 37°C | Homo sapiens | |
41.2 | - |
O2 | cosubstrate N-terminal Cys of RGS5, pH not specified in the publication, 37°C | Homo sapiens | |
56.6 | - |
O2 | cosubstrate N-terminal Cys of RGS4, pH not specified in the publication, 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | ADO catalyzes conversion of N-terminal cysteine to cysteine sulfinic acid and is related to the plant cysteine oxidases that mediate responses to hypoxia by an identical post-translational modification. In human cells ADO regulates the RGS4/5 (regulator of G-protein signalling) N-degron substrates, modulates G-protein coupled Ca2+ signals and MAPK activity, and acts on N-terminal cysteine proteins including the angiogenic cytokine IL-32. Inactivation of ADO leads to constitutive upregulation of endogenous and transfected RGS4 and RGS5 proteins irrespective of oxygen levels | Homo sapiens |