Crystallization (Comment) | Organism |
---|---|
structures of both wild-type and engineered CDO variants containing unnatural tyrosines | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme contains a posttranslationally generated Cys93-Tyr157 crosslinked cofactor. Incorporating unnatural tyrosines in place of Tyr157 via a genetic method gives catalytically active variants with a thioether bond between Cys93 and the halogen-substituted Tyr157. Crystal structures and data of both wild-type and engineered CDO variants in the purely uncrosslinked form and with a mature cofactor indicate that the enzyme can catalyze oxidative C-F or C-Cl bond cleavage, resulting in a substantial conformational change of both Cys93 and Tyr157 during cofactor assembly | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16878 | - |
- |