Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Carboxyethyl-L-cysteine | at 10 mM, activation | Rattus norvegicus | |
carboxymethyl-L-cysteine | at 10 mM, activation | Rattus norvegicus | |
cysteamine | at 100 mM, activation | Rattus norvegicus | |
D-cysteine | at 10 mM, activation | Rattus norvegicus | |
DL-homocysteine | at 100 mM, slight activation | Rattus norvegicus | |
hydrocortisone | induces | Rattus norvegicus | |
methionine | induces | Rattus norvegicus | |
N-acetyl-L-cysteine | at 100 mM, slight activation | Rattus norvegicus | |
NAD(P)H | stimulation | Rattus norvegicus | |
S-methyl-L-cysteine | at 10 mM, activation | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Carboxyethyl-L-cysteine | 53% inhibition at 1 mM | Rattus norvegicus | |
carboxymethyl-L-cysteine | 37% inhibition at 1 mM | Rattus norvegicus | |
cysteamine | 39% inhibition at 10 mM | Rattus norvegicus | |
D-cysteine | inhibition at 1 mM | Rattus norvegicus | |
DL-homocysteine | - |
Rattus norvegicus | |
N-acetyl-L-cysteine | 35% inhibition at 10 mM | Rattus norvegicus | |
S-methyl-L-cysteine | 34% inhibition at 1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
cysteine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | Fe2+ required, 0.8-0.9 gatom of iron per 22500 g of purified protein | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
SDS-PAGE, gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
rapid and irreversible inactivation under aerobic conditions, inactivation can be prevented by a distinct cytoplasmic protein, i.e. protein A | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
using acetone fractionation, column chromatography on DEAE-cellulose, Sephadex G-100, hydroxylapatite, DEAE-Sephadex A-25 and Sephadex G-75 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, no significant loss of activity after 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | highly specific for L-cysteine | Rattus norvegicus | 3-sulfino-L-alanine | - |
? | |
additional information | L-cystine, D-cysteine, carboxymethyl-L-cysteine, carboxyethyl-L-cysteine, S-methyl-L-cysteine, N-acetyl-L-cysteine, DL-homocysteine and cysteamine do not serve as substrates | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 22500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |