Literature summary for 1.12.7.2 extracted from

Stripp, S.T.; Happe, T.
How algae produce hydrogen--news from the photosynthetic hydrogenase (2009), Dalton Trans., 2009, 9960-9969.

Metals/Ions

Metals/Ions	Comment	Organism
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Chlamydomonas reinhardtii
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Tetradesmus obliquus
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Clostridium pasteurianum
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	[Chlorella] fusca
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Megasphaera elsdenii
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Desulfovibrio desulfuricans
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Clostridium acetobutylicum
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Chlamydomonas moewusii
Fe2+	the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster	Chlorococcum submarinum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14000	-	1 * 46100 + 1 * 14000, calculated from amino acid sequence	Desulfovibrio desulfuricans
44600	-	calculated from amino acid sequence	Tetradesmus obliquus
45100	-	calculated from amino acid sequence	[Chlorella] fusca
45300	-	calculated from amino acid sequence	Chlorococcum submarinum
45400	-	calculated from amino acid sequence	Chlamydomonas moewusii
46100	-	1 * 46100 + 1 * 14000, calculated from amino acid sequence	Desulfovibrio desulfuricans
47300	-	isozyme HydA2, calculated from amino acid sequence	Chlamydomonas reinhardtii
47500	-	isozyme HydA1, calculated from amino acid sequence	Chlamydomonas reinhardtii
48000	-	isozyme HydA1, SDS-PAGE	Chlamydomonas reinhardtii
53600	-	calculated from amino acid sequence	Megasphaera elsdenii
63800	-	calculated from amino acid sequence	Clostridium pasteurianum
64300	-	calculated from amino acid sequence	Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
H+ + reduced ferredoxin	Chlamydomonas reinhardtii	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Tetradesmus obliquus	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Clostridium pasteurianum	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	[Chlorella] fusca	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Megasphaera elsdenii	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Desulfovibrio desulfuricans	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Clostridium acetobutylicum	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Chlamydomonas moewusii	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	Chlorococcum submarinum	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	H2 + oxidized ferredoxin	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas moewusii	-	-	-
Chlamydomonas reinhardtii	-	-	-
Chlorococcum submarinum	-	-	-
Clostridium acetobutylicum	-	-	-
Clostridium pasteurianum	-	-	-
Desulfovibrio desulfuricans	-	-	-
Megasphaera elsdenii	-	-	-
Tetradesmus obliquus	-	-	-
[Chlorella] fusca	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity column chromatography with immobilised ferredoxin	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Chlamydomonas reinhardtii	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Tetradesmus obliquus	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Clostridium pasteurianum	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	[Chlorella] fusca	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Megasphaera elsdenii	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Desulfovibrio desulfuricans	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Clostridium acetobutylicum	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Chlamydomonas moewusii	H2 + oxidized ferredoxin	-	?
H+ + reduced ferredoxin	hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin	Chlorococcum submarinum	H2 + oxidized ferredoxin	-	?
H+ + reduced methyl viologen	-	Chlamydomonas reinhardtii	H2 + oxidized methyl viologen	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 46100 + 1 * 14000, calculated from amino acid sequence	Desulfovibrio desulfuricans

Synonyms

Synonyms	Comment	Organism
CpI	-	Clostridium pasteurianum
DDH	-	Desulfovibrio desulfuricans
hydA	-	Tetradesmus obliquus
hydA	-	[Chlorella] fusca
hydA	-	Megasphaera elsdenii
hydA	-	Clostridium acetobutylicum
hydA	-	Chlorococcum submarinum
HydA1	-	Chlamydomonas moewusii
HydA1	isozyme	Chlamydomonas reinhardtii
HydA2	isozyme	Chlamydomonas reinhardtii
hydrogenase of the Fe-only type	-	Chlamydomonas reinhardtii
[FeFe] hydrogenase	-	Chlamydomonas reinhardtii
[FeFe] hydrogenase	-	Tetradesmus obliquus
[FeFe] hydrogenase	-	Clostridium pasteurianum
[FeFe] hydrogenase	-	[Chlorella] fusca
[FeFe] hydrogenase	-	Megasphaera elsdenii
[FeFe] hydrogenase	-	Desulfovibrio desulfuricans
[FeFe] hydrogenase	-	Clostridium acetobutylicum
[FeFe] hydrogenase	-	Chlamydomonas moewusii
[FeFe] hydrogenase	-	Chlorococcum submarinum

Cofactor

Cofactor	Comment	Organism
Ferredoxin	-	Chlamydomonas reinhardtii
Ferredoxin	-	Tetradesmus obliquus
Ferredoxin	-	Clostridium pasteurianum
Ferredoxin	-	[Chlorella] fusca
Ferredoxin	-	Megasphaera elsdenii
Ferredoxin	-	Desulfovibrio desulfuricans
Ferredoxin	-	Clostridium acetobutylicum
Ferredoxin	-	Chlamydomonas moewusii
Ferredoxin	-	Chlorococcum submarinum

Expression

Organism	Comment	Expression
Chlamydomonas reinhardtii	activation and de novo synthesis of the protein is inhibited by cycloheximide but not chloramphenicol	up