Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains at least one [4Fe4S]+ and at least one [2Fe2S]+ cluster | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + NADH | Synechocystis sp. | - |
H2 + NAD+ | - |
? | |
H+ + NADPH | Synechocystis sp. | - |
H2 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | six accessory proteins HypA-F (hydrogenase pleiotropic proteins) and one protease (HoxW) are necessary for post-translational processing of the bidirectional [NiFe] hydrogenase | Synechocystis sp. |
Purification (Comment) | Organism |
---|---|
Strep-Tactin-Sepharose column chromatography | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.076 | - |
crude extract, pH and temperature not specified in the publication | Synechocystis sp. |
87.78 | - |
after 1155old purification, pH and temperature not specified in the publication | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + NADH | - |
Synechocystis sp. | H2 + NAD+ | - |
? | |
H+ + NADPH | - |
Synechocystis sp. | H2 + NADP+ | - |
? | |
H+ + reduced dithionite | - |
Synechocystis sp. | H2 + oxidized dithionite | - |
? | |
H+ + reduced methyl viologen | - |
Synechocystis sp. | H2 + oxidized methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bidirectional [NiFe] hydrogenase | - |
Synechocystis sp. |
Hox | - |
Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Synechocystis sp. | |
NADPH | - |
Synechocystis sp. |